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Immunological screening of Lassa Virus among Health workers and Contacts of patients of Lassa fever in Ondo State
Immunobiology ( IF 2.5 ) Pub Date : 2021-03-02 , DOI: 10.1016/j.imbio.2021.152076
Joseph Ojonugwa Shaibu 1 , Olumuyiwa Babalola Salu 2 , Olufemi Samuel Amoo 1 , Ifeoma Idigbe 3 , Adesola Zaidat Musa 3 , Oliver Chukwujekwu Ezechi 3 , Chukwuyem Abejegah 4 , Oluwafemi Ayodeji 4 , Babatunde Lawal Salako 3 , Sunday Aremu Omilabu 5 , Rosemary Ajuma Audu 1
Affiliation  

Background

The increasing trends of morbidity and mortality of Lassa fever is becoming more alarming in Nigeria. Information about immune response to the virus is limited. At exposure, the level of immunity plays a vital role in the vulnerability of individuals infected.

Objective

Investigating the immune status of health workers, infected cases and contacts of infected cases of Lassa fever in Ondo State.

Study Design

Blood samples were collected from 233 individuals comprising 102 health workers, 22 infected cases and 109 contacts of infected cases from Owo and Ose Local Government Areas and transported in triple level packaging. Plasma samples were analyzed for IgG and IgM markers using ReLASV® Pan-Lassa NP IgG/IgM ELISA Kit (Zalgen Labs, LLC, USA) while RNAs extracted from IgM positive samples were analyzed for LASV RNA according to manufacturers’ instructions.

Result

Among the health workers, 20/102 (19.6%) and 2/102 (2.0%) were IgG and IgM positive respectively. While 16/22 (72.7%) and 14/22 (63.6%) were IgG and IgM positive respectively among the infected cases. Of the contacts of infected cases screened, 64/109 (58.7%) were IgG positive while 4/109 (3.7%) were positive for IgM. There was no detectable LASV RNA in the samples analyzed.

Conclusion

These findings suggest that majority of the health workers are naïve to the virus and hence may be prone to the viral infection. It could also be suggestive that a good personal protective procedure is been practiced by the health workers, hence the low exposure. However, most of the contacts of infected cases show exposure to the virus.



中文翻译:

翁多州卫生工作者和拉沙热患者接触者中的拉沙病毒免疫学筛查

背景

拉沙热发病率和死亡率的上升趋势在尼日利亚变得更加令人担忧。关于对病毒的免疫反应的信息是有限的。在暴露时,免疫水平在受感染个体的脆弱性中起着至关重要的作用。

客观的

调查翁多州的卫生工作者、感染病例和拉沙热感染病例的接触者的免疫状况。

学习规划

从 Owo 和 Ose 地方政府地区的 233 人中采集了血液样本,其中包括 102 名卫生工作者、22 名感染病例和 109 名感染病例的接触者,并以三层包装运输。使用 ReLASV® Pan-Lassa NP IgG/IgM ELISA 试剂盒(Zalgen Labs, LLC, USA)分析血浆样品的 IgG 和 IgM 标志物,同时根据制造商的说明分析从 IgM 阳性样品中提取的 RNA 的 LASV RNA。

结果

在卫生工作者中,20/102 (19.6%) 和 2/102 (2.0%) 分别为 IgG 和 IgM 阳性。而感染病例中IgG和IgM分别为16/22(72.7%)和14/22(63.6%)。在筛查的感染病例接触者中,64/109 (58.7%) 为 IgG 阳性,4/109 (3.7%) 为 IgM 阳性。在分析的样品中没有可检测到的 LASV RNA。

结论

这些发现表明,大多数卫生工作者对病毒不敏感,因此可能容易感染病毒。这也可能表明卫生工作者实施了良好的个人防护程序,因此接触率低。但是,大多数感染病例的接触者都暴露于病毒。

更新日期:2021-03-07
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