The assessment of phenotypic variation across populations can help to select genotypes that are potentially resistant to the ongoing and predicted climatic variations. Phenotypic variation is expressed as morphological, physiological, or metabolic changes in response to environmental factors. Agave mapisaga and A. salmiana populations exhibit morphological variation regarding their geographical distribution and due to intense anthropogenic influence. This study aimed to identify metabolic traits in young plants of A. mapisaga and A. salmiana, involved in plant resistance to temperature stress, which can help to understand differences between provenances and between species. Agave plants propagated from four seed origins, collected from three “pulque” (a fermented beverage) production areas in the state of Hidalgo, Mexico, were assessed. Four-month-old plants of each seed origin (species*provenance) were maintained during one month in growth chambers, at day/night temperature (T) regimes for 12/12 h: (1) 25/25T, (2) 25/15T, and (3) 25/15 plus 45 °C T for 4 h between 11 am and 3 pm (45T). The plant dry biomass, maximum quantum efficiency of PSII photochemistry (Fv/Fm), total free amino acids (TFAA), total free phenol (TFP), and free soluble sugar concentrations were measured. Minimum values for dry biomass, TFP, glucose, and sucrose concentrations occurred in the 25/25T regime. Plant biomass, and TFAA and glucose concentrations were differently affected by seed provenances; but Fv/Fm rate, or the TFP, fructose, and sucrose concentrations had no relation to seed provenance. Moderate high night temperatures drastically affected the growth and metabolism of A. mapisaga and A. salmiana. In conclusion, the response to different temperature stress treatments depends partly on species, but less on seed provenance.

跨人群表型变异的评估可以帮助选择可能对正在进行的和预测的气候变异具有抗性的基因型。表型变异表示为响应环境因素的形态，生理或代谢变化。龙舌兰Mapisaga和A. salmiana种群的地理分布和强烈的人为影响都表现出形态上的变异。这项研究旨在确定与植物对温度胁迫的抗性有关的mapisaga和salmiana年轻植物的代谢特征，这可以帮助理解种源之间和物种之间的差异。龙舌兰对从墨西哥Hidalgo州的三个“发酵食品”生产区收集的四个种子起源繁殖的植物进行了评估。每个种子起源（物种*种源）的四个月大的植物在生长室中维持一个月的昼夜温度（T），持续12/12小时：（1）25 / 25T，（2）25 / 15T，以及（3）25/15加上45°CT，在上午11点至下午3点（45T）之间持续4小时。测量了植物干生物量，PSII光化学的最大量子效率（Fv / Fm），总游离氨基酸（TFAA），总游离酚（TFP）和游离可溶性糖浓度。干生物量，TFP，葡萄糖和蔗糖浓度的最小值出现在25 / 25T方案中。种子来源对植物生物量，TFAA和葡萄糖浓度的影响不同。但Fv / Fm率或TFP（果糖）蔗糖浓度与种子出处无关。适中的高温严重影响了黑麦草的生长和代谢A. mapisaga和A. salmiana。总之，对不同温度胁迫处理的响应部分取决于物种，而较少取决于种子出处。