Exposure to oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) at critical developmental time-points in fish models impairs red blood cell concentrations in a regioselective manner, with 2-hydroxychrysene being more potent than 6-hydroxychrysene. To better characterize this phenomenon, embryos of Japanese medaka (Oryzias latipes) were exposed to 2- or 6-hydroxychrysene (0.5, 2, or 5 μM) from 4 h-post-fertilization (hpf) to 7 d-post-fertilization. Following exposure, hemoglobin concentrations were quantified by staining fixed embryos with o-dianisidine (a hemoglobin-specific dye) and stained embryos were imaged using brightfield microscopy. Exposure to 2-hydroxychrysene resulted in a concentration-dependent decrease in hemoglobin relative to vehicle-exposed embryos, while only the highest concentration of 6-hydroxychrysene resulted in a significant decrease in hemoglobin. All tested concentrations of 2-hydroxychrysene also caused significant mortality (12.2 % ± 2.94, 38.9 % ± 14.4, 85.6 % ± 11.3), whereas mortality was not observed following exposure to 6-hydroxychrysene. Therefore, treatment of embryos with 2-hydroxychrysene at various developmental stages and durations was subsequently conducted to identify key developmental landmarks that may be targeted by 2-hydroxychrysene. A sensitive window of developmental toxicity to 2-hydroxychrysene was found between 52–100 hpf, with a 24 h exposure to 10 μM 2-hydroxychrysene resulting in significant anemia and mortality. Since exposure to 2-hydroxychrysene from 52 to 100 hpf, a window that includes liver morphogenesis in medaka, resulted in the highest magnitude of toxicity, liver development and function may have a role in 2-hydroxychrysene developmental toxicity.

在鱼类模型的关键发育时间点接触含氧多环芳烃（oxy-PAHs），会以区域选择性的方式损害红细胞的浓度，其中2-羟基ch比6-羟基ch更有效。为了更好地表征这种现象，从受精后4 h（hpf）到受精后7 d ，将日本（Oryzias latipes）的胚暴露于2-或6-羟基ch（0.5、2或5μM）。暴露后，血红蛋白浓度通过用o染色固定的胚胎来定量-dianisidine（血红蛋白特异性染料）和染色的胚胎使用明场显微镜成像。相对于赋形剂暴露的胚胎，暴露于2-羟基hydroxy导致血红蛋白浓度依赖性降低，而只有最高浓度的6-羟基ch导致血红蛋白显着降低。所有测试浓度的2-羟基ch也引起显着的死亡率（12.2％±2.94，38.9％±14.4，85.6％±11.3），而暴露于6-羟基ch后未观察到死亡率。因此，随后在各个发育阶段和持续时间用2-羟基hydroxy处理胚胎，以鉴定可能被2-羟基ch靶向的关键发育标志。在52–100 hpf之间发现了对2-羟基hydroxy的发育毒性的敏感窗口，暴露于10μM2-羟基丙烯24小时会导致明显的贫血和死亡率。由于暴露于52至100 hpf的2-羟基ch（包括在medaka中的肝脏形态发生）的窗口会导致最高程度的毒性，因此肝脏的发育和功能可能在2-羟基ch的发育毒性中起作用。