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Expression patterns of ASR1 , PIN2, and PAL genes in tomato and eggplant after treatment with different inducers
International Journal of Tropical Insect Science ( IF 1.2 ) Pub Date : 2021-03-02 , DOI: 10.1007/s42690-021-00476-w
Fatemeh Jafarbeigi , Mohammad Amin Samih , Hossein Alaei

Salicylic acid (SA) and β-aminobutyric acid (BABA) are signaling compounds that regulate a large number of defense responses in plants after exposure to the biotic and abiotic stresses. The antagonistic fungus Trichoderma harzianum is well-known as a potential biocontrol agent that also induces defensive responses. In the present study, we tested the up- and down-regulation of defense responses of eggplant and tomato mediated by root application of SA and BABA as well as T. harzianum. Especially, we analyzed and compared the expression of ASR1, PIN2, and PAL in 4, 10, and 14 d post treatment. In all time courses, the root Trichoderma application + root β-aminobutyric acid application (RT + RBABA)- and root Trichoderma application + root salicylic acid application (RT + RSA)-treated plants had a much higher expression of genes than those on RT-treated plants and controls. Also, control and treated plants had significantly higher expression of ASR1 and PIN2 on the 4th day compared to other time courses. The PAL gene expression reached the maximum level at 10 days after treatment and it increased to about twofold level than 4 days after treatment. Information on expression profiles of the genes in different plants and under various inducers helps to identify candidates to the increase of resistance to environmental stress conditions.



中文翻译:

不同诱导剂处理后番茄和茄子中ASR1,PIN2和PAL基因的表达模式

水杨酸(SA)和β-氨基丁酸(BABA)是信号传导化合物,可在暴露于生物和非生物胁迫后调节植物中的大量防御反应。拮抗真菌哈茨木霉Trichoderma harzianum)是一种潜在的生物防治剂,众所周知,它还可以诱导防御反应。在本研究中,我们测试了SA和BABA以及哈茨木霉的根部施用介导的茄子和番茄防御反应的上调和下调。特别是,我们分析并比较了ASR1PIN2PAL在治疗后4、10和14 d的表达。在所有时间课程中,根木霉菌应用程序+ rootβ-氨基丁酸施用(RT + RBABA)和根木霉施用+根水杨酸施用(RT + RSA)处理过的植物的基因表达比RT处理过的植物和对照高得多。此外,控制和处理的植物具有更高的显著表达ASR1PIN2在4相对于其他时间进程天。在PAL的基因表达,在治疗后10天达到最高水平,并将其处理后增加至约两倍水平超过4天。有关在不同植物中和在各种诱导物下的基因表达谱的信息有助于鉴定候选物,以增加对环境胁迫条件的抗性。

更新日期:2021-03-02
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