The African elephant (Loxodonta africana) is listed as vulnerable, with wild populations threatened by habitat loss and poaching. Clinical pathology is used to detect and monitor disease and injury, however existing reference interval (RI) studies for this species have been performed with outdated analytical methods, small sample sizes or using only managed animals. The aim of this study was to generate hematology and clinical chemistry RIs, using samples from the free-ranging elephant population in the Kruger National Park, South Africa. Hematology RIs were derived from EDTA whole blood samples automatically analyzed (n = 23); manual PCV measured from 48 samples; and differential cell count results (n = 51) were included. Clinical chemistry RIs were generated from the results of automated analyzers on stored serum samples (n = 50). Reference intervals were generated according to American Society for Veterinary Clinical Pathology guidelines with a strict exclusion of outliers. Hematology RIs were: PCV 34–49%, RBC 2.80–3.96 × 10¹²/L, HGB 116–163 g/L, MCV 112–134 fL, MCH 35.5–45.2 pg, MCHC 314–364 g/L, PLT 182–386 × 10⁹/L, WBC 7.5–15.2 × 10⁹/L, segmented heterophils 1.5–4.0 × 10⁹/L, band heterophils 0.0–0.2 × 10⁹/L, total monocytes 3.6–7.6 × 10⁹/L (means for “regular” were 35.2%, bilobed 8.6%, round 3.9% of total leukocytes), lymphocytes 1.1–5.5 × 10⁹/L, eosinophils 0.0–0.9 × 10⁹/L, basophils 0.0–0.1 × 10⁹/L. Clinical chemistry RIs were: albumin 41–55 g/L, ALP 30–122 U/L, AST 9–34 U/L, calcium 2.56–3.02 mmol/L, CK 85–322 U/L, GGT 7–16 U/L, globulin 30–59 g/L, magnesium 1.15–1.70 mmol/L, phosphorus 1.28–2.31 mmol/L, total protein 77–109 g/L, urea 1.2–4.6 mmol/L. Reference intervals were narrower than those reported in other studies. These RI will be helpful in the future management of injured or diseased elephants in national parks and zoological settings.

非洲大象（非洲象）被列为脆弱人群，其野生种群受到栖息地丧失和偷猎的威胁。临床病理学用于检测和监测疾病和损伤，但是，该物种的现有参考间隔（RI）研究已经使用过时的分析方法，小样本量或仅使用受管理的动物来进行。这项研究的目的是使用来自南非克鲁格国家公园自由放养的大象种群的样本来产生血液学和临床化学RI。血液学RIs来自自动分析的EDTA全血样品（ñ= 23）; 从48个样本中测量的手动PCV；和差分细胞计数结果（ñ= 51）。临床化学RI是由自动分析仪对储存的血清样品的结果生成的（ñ= 50）。参考间隔是根据美国兽医临床病理学会指南生成的，严格排除了异常值。血液学RIs为：PCV 34–49％，RBC 2.80–3.96×10 ¹² / L，HGB 116–163 g / L，MCV 112–134 fL，MCH 35.5–45.2 pg，MCHC 314–364 g / L，PLT 182 –386×10 ⁹ / L，WBC 7.5–15.2×10 ⁹ / L，分段异嗜性1.5–4.0×10 ⁹ / L，条带异嗜性0.0–0.2×10 ⁹ / L，单核细胞总数3.6–7.6×10 ⁹ / L （用于“常规”分别为35.2％，8.6 bilobed％，总白细胞的圆3.9％），淋巴细胞1.1-5.5×10 ⁹ / L，嗜酸性粒细胞0.0-0.9×10 ⁹ / L，嗜碱性粒细胞0.0-0.1×10 ⁹/升 临床化学RIs为：白蛋白41–55 g / L，ALP 30–122 U / L，AST 9–34 U / L，钙2.56–3.02 mmol / L，CK 85–322 U / L，GGT 7–16 U / L，球蛋白30–59 g / L，镁1.15–1.70 mmol / L，磷1.28–2.31 mmol / L，总蛋白77–109 g / L，尿素1.2–4.6 mmol / L。参考区间比其他研究报道的要窄。这些RI将有助于将来在国家公园和生态环境中管理受伤或患病的大象。