Small RNAs (sRNAs) are essential regulatory molecules, and there are three major sRNA classes in plants: microRNAs (miRNAs), phased small interfering RNAs (phased siRNAs or phasiRNAs), and heterochromatic siRNAs (hc-siRNAs). Excluding miRNAs, the other two classes are not well annotated or available in public databases for most sequenced plant genomes. We performed a comprehensive sRNA annotation of 143 plant species that have fully sequenced genomes and next-generation sequencing sRNA data publicly available. The results are available via an online repository called sRNAanno (www.plantsRNAs.org). Compared with other public plant sRNA databases, we obtained was much more miRNA annotations, which are more complete and reliable because of the consistent and highly stringent criteria used in our miRNA annotations. sRNAanno also provides free access to genomic information for >22,721 PHAS loci and >22 million hc-siRNA loci annotated from these 143 plant species. Both miRNA and PHAS loci can be easily browsed to view their main features, and a collection of archetypal trans-acting siRNA 3 (TAS3) genes were annotated separately for quick access. To facilitate the ease of sRNA annotation, sRNAanno provides free service for sRNA annotations to the community. In summary, the sRNAanno database is a great resource to facilitate genomic and genetic research on plant small RNAs.

中文翻译：

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sRNAanno——植物中统一注释的小 RNA 的数据库存储库

小 RNA (sRNA) 是必不可少的调节分子，植物中存在三种主要的 sRNA 类别：microRNA (miRNA)、定相小干扰 RNA（定相 siRNA 或 phasiRNA）和异染色质 siRNA (hc-siRNA)。除 miRNA 外，其他两类在大多数已测序植物基因组的公共数据库中都没有得到很好的注释或可用。我们对 143 个植物物种进行了全面的 sRNA 注释，这些植物物种具有完全测序的基因组和公开的下一代测序 sRNA 数据。结果可通过名为 sRNAanno 的在线存储库获得（www.plantsRNAs.org）。与其他公共植物 sRNA 数据库相比，我们获得了更多的 miRNA 注释，这些注释更加完整和可靠，因为我们的 miRNA 注释中使用了一致且高度严格的标准。sRNAanno 还为 >22,721 提供免费的基因组信息访问相从这 143 个植物物种中注释的基因座和 > 2200 万个 hc-siRNA 基因座。miRNA 和相可以轻松浏览基因座以查看其主要特征，并收集原型反式作用siRNA 3(TAS3) 基因被单独注释以便快速访问。为了方便 sRNA 注释，sRNAanno 向社区提供免费的 sRNA 注释服务。总之，sRNAanno 数据库是促进植物小 RNA 基因组和遗传研究的重要资源。