Abstract

The persistence of Salmonella enterica on abiotic surfaces in hospitals and the agri-food industries leads to several infections worldwide. In this context, this work aimed to study the adhesion of S. Enteritidis on plasma-modified stainless steel to prevent biofilm-associated-infections. Surface modification was achieved by the elaboration of organosilicon coatings from the monomer 1,1,3,3-tetramethyldisiloxane, mixed with oxygen, using a flowing nitrogen microwave post-discharge plasma polymerization technique. The effect of cold plasma parameters on the properties of the coatings, the coated surface topography and S. Enteritidis cell adhesion was studied. The results showed that the surface topography influenced the bacterial adhesion rate. Indeed, rough surfaces did not repel S. Enteritidis since the number of attached cells on these coatings was between 30 ± 4 to 65 ± 4 bacteria per microscopic field. Otherwise, smoother surfaces demonstrated an anti-adhesive character since the number of attached cells was almost nil on these coatings.

摘要

肠沙门氏菌在医院和农业食品行业的非生物表面上的持久性导致全球数种感染。在这方面，这个工作的目的是研究的粘附S.上等离子体改性不锈钢肠炎，以防止生物膜相关的感染。表面改性是通过使用流动的氮气微波后放电等离子体聚合技术，从与氧混合而成的单体1,1,3,3-四甲基二硅氧烷精制有机硅涂层而实现的。冷等离子体参数对涂层性能，涂层表面形貌和S的影响研究肠炎小肠细胞粘附。结果表明，表面形貌影响细菌的附着率。实际上，粗糙表面不排斥S.肠炎因为附着的细胞对这些涂层的数量是每显微镜视野菌30±65 4之间4至±。否则，由于在这些涂层上附着的细胞数几乎为零，因此较光滑的表面表现出抗粘合特性。