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Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-28 , DOI: 10.1002/arch.21776 Elena V. Burdina 1 , Roman A. Bykov 1 , Petr N. Menshanov 1, 2, 3 , Yury Yu. Ilinsky 1 , Nataly Е. Gruntenko 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-28 , DOI: 10.1002/arch.21776 Elena V. Burdina 1 , Roman A. Bykov 1 , Petr N. Menshanov 1, 2, 3 , Yury Yu. Ilinsky 1 , Nataly Е. Gruntenko 1
Affiliation
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Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine‐mediated.
中文翻译：
独特的Wolbachia菌株wMelPlus增强了果蝇的抗热应激能力
母系内生共生细菌沃尔巴克氏菌感染了全球果蝇果蝇。它的遗传多样性包括几种密切相关的基因型，这可以归因于两个主要的基因型组：wMel和wMelCS。在这里，我们研究了八种D. melanogaster品系，它们带有野生型杂交Bi90品系的核背景和具有或不具有不同起源的Wolbachia的细胞质背景，每个都属于wMelCS基因型组。我们分析了这七种Wolbachia菌株对D. melanogaster的抗热胁迫性和多巴胺代谢的影响女性。仅在感染了wMelPlus菌株细菌的品系中，增加了在热胁迫下（38°C，3 h 30 min）的存活率。同时，在正常条件下，感染了所有研究菌株的雌性中的碱性磷酸酶（一种调节多巴胺前体酪氨酸池的酶）的活性增加，并保留了未感染品系典型的对热胁迫的响应。因此，我们发现了独特的Wolbachia菌株，可以增加宿主抗逆性，并证明这种抗性的机制不是多巴胺介导的。
更新日期：2021-04-01
中文翻译：
独特的Wolbachia菌株wMelPlus增强了果蝇的抗热应激能力
母系内生共生细菌沃尔巴克氏菌感染了全球果蝇果蝇。它的遗传多样性包括几种密切相关的基因型，这可以归因于两个主要的基因型组：wMel和wMelCS。在这里，我们研究了八种D. melanogaster品系，它们带有野生型杂交Bi90品系的核背景和具有或不具有不同起源的Wolbachia的细胞质背景，每个都属于wMelCS基因型组。我们分析了这七种Wolbachia菌株对D. melanogaster的抗热胁迫性和多巴胺代谢的影响女性。仅在感染了wMelPlus菌株细菌的品系中，增加了在热胁迫下（38°C，3 h 30 min）的存活率。同时，在正常条件下，感染了所有研究菌株的雌性中的碱性磷酸酶（一种调节多巴胺前体酪氨酸池的酶）的活性增加，并保留了未感染品系典型的对热胁迫的响应。因此，我们发现了独特的Wolbachia菌株，可以增加宿主抗逆性，并证明这种抗性的机制不是多巴胺介导的。
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