To realize the potential of microalgae in the biorefinery context, exploitation of multiple products is necessary for profitability and bioproduct valorization. Appropriate analytical tools are required for growth optimization, culture monitoring, and quality control purposes, with safe, low‐tech, and low‐cost solutions favorable. Rapid, high‐throughput, and user‐friendly methodologies were devised for (a) determination of phycobiliproteins, chlorophylls, carotenoids, proteins, carbohydrates, and lipids and (b) qualitative and quantitative carotenoid profiling using UPLC‐PDA‐MS^E. The complementary methods were applied on 11 commercially important microalgal strains including prasinophytes, haptophytes, and cyanobacteria, highlighting the suitability of some strains for coproduct exploitation and the method utility for research and industrial biotechnology applications. The UPLC method allowed separation of 41 different carotenoid compounds in <15 min. Simple techniques are described for further quantification and comparison of pigment profiles, allowing for easy strain selection and optimization for pigment production, with suitability for biotechnological or biomedical applications.

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微藻和蓝藻生物精炼的快速分析方法：在具有工业重要性的菌株上的应用

为了实现微藻在生物炼制环境中的潜力，开发多种产品对于盈利能力和生物产品价值化是必要的。生长优化、培养监测和质量控制需要适当的分析工具，安全、低技术和低成本的解决方案是有利的。设计了快速、高通量和用户友好的方法，用于 (a) 测定藻胆蛋白、叶绿素、类胡萝卜素、蛋白质、碳水化合物和脂质，以及 (b) 使用 UPLC-PDA-MS ^{E 进行}类胡萝卜素定性和定量分析. 补充方法应用于 11 种具有商业重要性的微藻菌株，包括草本植物、触手植物和蓝藻，突出了一些菌株用于副产品开发的适用性以及研究和工业生物技术应用的方法效用。UPLC 方法允许在 <15 分钟内分离 41 种不同的类胡萝卜素化合物。描述了用于进一步量化和比较色素分布的简单技术，允许轻松选择和优化色素生产，适用于生物技术或生物医学应用。