We have reported the formation of fluorescent CdSe/ZnS quantum dots (QDs) incorporated poly (styrene-co-maleic anhydride) spheres in nanoscale range with a simple emulsification solvent evaporation strategy. The influence parameters on the size and internal structure of the composite nanospheres, such as copolmer/QDs mass ratio and volume ratio of water/chloroform were systematically investigated. Our obtained QDs incorporated polymer nanospheres were suitable for some immunoassays which strongly required nano-sized fluorescent labels, such as lateral flow immunoassays (LFIAs), in which “easily flowing chromatographically along the strip” was needed. Finally, carboxyl-functionalized nanospheres were used as fluorescent tags and imported into LFIA system to on-site high-sensitively assess the level of C-reaction protein (CRP). Within the range of high-sensitive detection (from 0.04 to 10.0 mg/L), nanosphere-based LFIA sensor exhibited linear detection of CRP (R² = 0.9995) and a detection limit of 0.04 mg/L, which may be useful to improve the risk assessment of cardiovascular disease.

我们已经报道荧光的CdSe / ZnS量子点（QD）掺入聚的形成（苯乙烯-共-马来酸酐）球，具有简单的乳化溶剂蒸发策略。系统研究了共聚单体/ QDs质量比和水/氯仿体积比对纳米复合球尺寸和内部结构的影响。我们获得的结合了QDs的聚合物纳米球适用于某些强烈需要纳米级荧光标记物的免疫测定，例如横向流动免疫测定（LFIA），其中需要“沿色谱条轻松流动”。最后，将羧基官能化的纳米球用作荧光标签，并将其导入LFIA系统中，以现场高灵敏度地评估C反应蛋白（CRP）的水平。在高灵敏度检测范围内（从0.04到10.0 mg / L），²  = 0.9995）和0.04 mg / L的检出限，可能有助于改善心血管疾病的风险评估。