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Elicitation Improves Phenolic Acid Content and Antioxidant Enzymes Activity in Salvia leriifolia Cell Cultures
Iranian Journal of Science and Technology, Transactions A: Science ( IF 1.4 ) Pub Date : 2021-03-01 , DOI: 10.1007/s40995-021-01070-y
Masoomeh Modarres , Mohammad Ehsan Taghavizadeh Yazdi

Elicitors, such as salicylic acid (SA), stand as the effective agents that are applied to plant cell suspension cultures for the fabrication of biomaterials. At this project, we have studied the elicitor effects on the increase in phenols, PAL activities, POX, and CAT enzymes that exist in the cell culture of Salvia leriifolia. Upon the addition of SA to the cell culture, an increase has been detected in the phenols (CA and Sal B) and the activity of PAL, POX, and CAT enzymes. The increases of phenols and PAL activity have been stimulated 48 h after being treated with SA and the activities of resulting antioxidant enzymes have been detected to be greatly improved. Elicitation of SA on phenols gathering has been dependent on the applied dose and time period. The appropriate SA assemblage for eliciting phenols accumulations has been determined to be 10 mg/L. Elicitation influence of SA on phenols gatherings has associated with the rate of PAL, indicating that PAL may be the crucial role for the synthesis of Sal B and CA. The increased PAL activity can lead to the progress of phenolic compounds amount, which is quite significant in regards to the usage of cell culture systems for biofabrication of secondary metabolites such as CA and Sal B.



中文翻译:

诱导提高丹参细胞培养物中的酚酸含量和抗氧化酶活性

水杨酸(SA)等引发剂是有效试剂,可应用于植物细胞悬浮培养物中以制造生物材料。在这个项目中,我们研究了激发因子对丹参细胞培养物中酚类,PAL活性,POX和CAT酶增加的影响。。将SA添加到细胞培养物中后,已发现酚(CA和Sal B)以及PAL,POX和CAT酶的活性增加。用SA处理后48小时刺激了酚类和PAL活性的增加,并且检测到所得抗氧化剂酶的活性大大提高。苯酚聚集对SA的启发取决于所施加的剂量和时间。确定引起苯酚积累的合适SA组合为10 mg / L。SA对酚类物质聚集的激发作用与PAL的发生率有关,这表明PAL可能是Sal B和CA合成的关键作用。增加的PAL活性可以导致酚类化合物量的增加,

更新日期:2021-03-01
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