Atmospheric pressure plasma jet (APPJ) is a promising technique for the sterilization of pathogenic microorganisms in an ambient environment. In this work, a helium-APPJ was generated by double dielectric barrier discharge and applied to the sterilization of model microorganism in air and water. Discharge characteristics (including waveform and frequency of applied voltage), jet properties (such as feed gas flow rate, jet length, thermal effect, and optic emission spectra), and sterilization performance (in terms of clear/sterilized area, size of plaques, and sterilization efficiency) were investigated. Homogeneous helium plasma jet was generated in an energy-efficient way (18 kHz, 6 kV, 0.08 W) with a 19 mm jet and limited heating. The He-APPJ achieved good sterilization performances within very short treatment time (as short as 30 s). For surface sterilization, the area of clear zone and size of the plaque were 1809 mm² and 48 mm, respectively, within 5 min treatment. For water sterilization, 99.8% sterilization efficiency was achieved within 5 min treatment. The optic emission spectra suggest that active species such as excited molecules, ions, and radicals were produced in the He-APPJ. The as-produced active species played important roles in the sterilization process.

中文翻译：

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He-Plasma Jet产生及其在大肠杆菌灭菌中的应用

大气压等离子体射流（APPJ）是一种在环境中对病原微生物进行灭菌的有前途的技术。在这项工作中，氦-APPJ是通过双重介电势垒放电产生的，并应用于空气和水中的模型微生物的灭菌。放电特性（包括施加电压的波形和频率），喷射特性（例如进料气流速，喷射长度，热效应和光学发射光谱）和灭菌性能（根据净/灭菌面积，噬菌斑大小，和杀菌效率）。均质氦等离子体射流以高能效方式（18 kHz，6 kV，0.08 W），19 mm射流和有限的加热产生。He-APPJ在极短的治疗时间内（短至30 s）获得了良好的灭菌性能。在5分钟内分别治疗²毫米和48毫米。对于水灭菌，在5分钟的处理时间内达到了99.8％的灭菌效率。光学发射光谱表明，He-APPJ中产生了活性物质，例如受激分子，离子和自由基。所产生的活性物质在灭菌过程中起着重要作用。