2,4,6-trichloroanisole (2,4,6-TCA) formation is often reported as a cause of taste and odor (T&O) problems in water distribution systems (WDSs). The biosynthesis via microbial O-methylation of 2,4,6-trichlorophenol (2,4,6-TCP) is the dominant formation pathway in distribution pipes. This paper attempted to utilize the reported data on the microbial O-methylation process to formulate deterministic kinetic models for explaining 2,4,6-TCA formation dynamics in WDSs. The pipe material’s critical role in stimulating O-methyltransferases enzymatic activity and regulating 2,4,6-TCP bioconversion in water was established. The kinetic expressions formulated were later applied to develop a novel EPANET-MSX-based multi-species reactive-transport (MSRT) model. The effects of operating conditions and temperature in directing the microbiological, chemical, and organoleptic quality variations in WDSs were analyzed using the MSRT model on two benchmark systems. The simulation results specified chlorine application’s implication in maintaining 2,4,6-TCA levels within its perception limit (4 ng/L). In addition, the temperature sensitivity of O-methyltransferases enzymatic activity was described, and the effect of temperature increase from 10 to 25 °C in accelerating the 2,4,6-TCA formation rate in WDSs was explained. Controlling source water 2,4,6-TCP concentration by accepting appropriate treatment techniques was recommended as the primary strategy for regulating the T&O problems in WDSs.

中文翻译：

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模拟2,4,6-三氯茴香醚（一种主要的味道和气味化合物）在水分配系统中的形成和传播

2,4,6-三氯茴香醚（2,4,6-TCA）的形成经常被报告为水分配系统（WDSs）中的味觉和气味（T＆O）问题的原因。2,4,6-三氯苯酚（2,4,6-TCP）通过微生物O-甲基化的生物合成是分配管道中的主要形成途径。本文试图利用有关微生物O-甲基化过程的报道数据来建立确定性动力学模型，以解释WDS中的2,4,6-TCA形成动力学。管道材料在刺激O中的关键作用-甲基转移酶的酶活性和调节水中2,4,6-TCP的生物转化已建立。制定的动力学表达式后来被用于开发一种新颖的基于EPANET-MSX的多物种反应转运（MSRT）模型。使用MSRT模型在两个基准系统上分析了操作条件和温度对指导WDS中微生物，化学和感官质量变化的影响。模拟结果说明了氯气应用在将2,4,6-TCA含量维持在其感知极限（4 ng / L）之内的意义。另外，O的温度敏感性描述了甲基转移酶的酶活性，并解释了温度从10升高到25°C对加速WDS中2,4,6-TCA形成速率的影响。建议通过接受适当的处理技术来控制源水2,4,6-TCP的浓度，作为调节WDS中T＆O问题的主要策略。