Abstract

In this study, diopside coated Poly-L-lactic acid-based (PLLA/Diopside) and Poly-L-lactic acid along with pluronic acid (PLLA-P123/Diopside) scaffolds were fabricated by electrospinning to improve the drawbacks with pure polymer and pure bioceramic scaffolds in bone tissue engineering application. The surface morphology, hydrophilicity, and mechanical strength were evaluated. The cell proliferation, differentiation, and expression of osteo related genes were evaluated by measuring the basic osteogenic markers and real time polymerase chain reaction, respectively. Diopside coated Poly-L-lactic acid-based nanofibrous scaffolds have improved surface and biological properties making them a good promising candidate for proliferation and osteogenic differentiation in bone repair and regeneration compared to nanofibrous scaffolds without diopside. Especially, post plasma treated diopside coated PLLA demonstrated better osteogenesis and expression of osteo related genes than the other groups. Although, diopside coated PLLA-P123 composite in comparison with PLLA-Plasma/diopside scaffolds were showed less osteogenesis and expression of osteo related genes, but the results were comparable. Therefore, diopside coated (PLLA-plasma and PLLA/P123 composite) scaffolds could be ideal cost effective grafts in bone repair and replacement therapy.

摘要

在这项研究中，通过静电纺丝制造透辉石涂层的聚-L-乳酸基（PLLA/透辉石）和聚-L-乳酸以及聚乳酸（PLLA-P123/透辉石）支架，以改善纯聚合物的缺点和纯生物陶瓷支架在骨组织工程中的应用。评估了表面形态、亲水性和机械强度。分别通过测量基本成骨标志物和实时聚合酶链反应来评估骨相关基因的细胞增殖、分化和表达。与不含透辉石的纳米纤维支架相比，透辉石涂层的聚-L-乳酸基纳米纤维支架具有改善的表面和生物学特性，使其成为骨修复和再生中增殖和成骨分化的良好候选者。特别是，经过等离子处理的透辉石涂层的 PLLA 表现出比其他组更好的成骨和骨相关基因的表达。尽管与 PLLA-Plasma/透辉石支架相比，透辉石涂层的 PLLA-P123 复合材料的成骨和骨相关基因的表达较少，但结果具有可比性。因此，透辉石涂层（PLLA 等离子和 PLLA/P123 复合材料）支架可能是骨修复和替代治疗中理想的成本有效的移植物。