ABSTRACT

Plant fermentation extracts (PFEs) produced from fermenting various fruits and vegetables by microorganisms have traditionally been consumed as healthy beverages in Asia. Herein, the immunomodulatory activities of a novel polysaccharide (AP) isolated from PFE were examined in RAW264.7 cells. The results showed that AP was composed of Rha, Xyl, Man and Glu in a molar ratio of 9.7:27.6:8.81:53.85 and the average molecular weight was 4.81 × 10⁵ Da. Our data showed that AP could significantly enhance the phagocytic activity of RAW264.7 cells and their ability to release NO, TNF-α, IL-6 and IL-10. Furthermore, AP could significantly up-regulate the mRNA expression of iNOS, cytokines (TNF-α, IL-6, IL-10) and pattern recognition receptors (TLR2, MR, GR, CR3 and SR). Western blotting demonstrated that the regulation of NO and TNF-α were mediated through the NF-κB and MAPK signalling pathways. These findings helped to elucidate the immunomodulatory properties of the polysaccharide AP and PFE.

摘要

传统上，通过微生物发酵各种水果和蔬菜而产生的植物发酵提取物（PFE）在亚洲已被用作健康饮料。本文中，在RAW264.7细胞中检查了从PFE分离的新型多糖（AP）的免疫调节活性。结果表明AP由Rha，Xyl，Man和Glu组成，摩尔比为9.7：27.6：8.81：53.85，平均分子量为4.81×10 ⁵ 大。我们的数据表明，AP可以显着增强RAW264.7细胞的吞噬活性及其释放NO，TNF-α，IL-6和IL-10的能力。此外，AP可以显着上调iNOS，细胞因子（TNF-α，IL-6，IL-10）和模式识别受体（TLR2，MR，GR，CR3和SR）的mRNA表达。Western blotting证实NO和TNF-α的调节是通过NF-κB和MAPK信号传导途径介导的。这些发现有助于阐明多糖AP和PFE的免疫调节特性。