ABSTRACT

A field experiment was conducted during two consecutive growing seasons (2017 and 2018) to evaluate the effects of inoculations with arbuscular mycorrhizal fungi (AMF) (Funneliformis mosseae, Rhizophagus intraradices and Claroideoglomus etunicatum) and phosphorus (P) fertilization (40 kg P₂O₅ ha⁻¹) on the growth, yield and P use efficiency (PUE) of two soybean (Glycine max L.) cultivars under low soil P (6 mg kg⁻¹) condition. AMF inoculation promotes the growth of soybean to some level compared to the P fertilized plot; however, inoculation (~960 spores per 100 g of soil) of R. intraradices or F. mosseae increased the number of nodules, nodule dry weight, shoot dry weight, root dry weight, number of pods, 100-seed weight and seed yield in both TGx 1440–1E and TGx 1448–2E soybean cultivars compared to C. etunicatum and the control. The P uptake in the shoot and grain in both cultivars increased with AMF inoculations in the order R. intraradices > F. mosseae > C. etunicatum. The studies showed that the highest yield response (44.2– 45.4%) and (44.9– 46.5%) over the control was revealed by the inoculation with R. intraradices and F. mosseae inoculants, respectively, in both soybean cultivars. TGx 1448–2E variety was more tolerant to low soil P availability and had higher root nodulation, seed yield and PUE than TGx 1448–2E variety. The results confirm functional variation among the three AMF isolates tested, which is crucial in establishing potential formulation of AMF inoculants to enhance soybean productivity in P-deficient soils.

摘要

在两个连续的生长季节（2017年和2018年）中进行了田间试验，以评估接种丛枝菌根真菌（AMF）（沙门氏菌，根瘤菌和Claroideoglomus etunicatum）以及磷（P）施肥（40 kg P ₂ O ₅ ha ^-1）对低土壤磷（6 mg kg ^-1）条件下两个大豆（Glycine max L.）品种的生长，产量和磷利用效率（PUE）的影响。与磷肥田相比，接种AMF可以使大豆的生长达到一定水平。但是，接种R. intraradices或F. mosseae（每100 g土壤约960孢子）增加结节，结节干重，地上部分干重，根干重，荚数，100-种子重量和种子产量在两个TGX 1440-1E和TGX 1448-2E大豆品种相比的数目C. etunicatum和控制。两种品种的芽和籽粒中的磷吸收量随接种AMF的增加依次为根内菌>苔藓（F. mosseae） >金钱草（C. etunicatum）。研究表明，接种R. intraradices和F. mosseae可以显示相对于对照的最高产量响应（44.2–45.4％）和（44.9–46.5％）两个大豆品种中分别接种了接种剂。TGx 1448-2E品种比TGx 1448-2E品种更耐低磷，根瘤，种子产量和PUE更高。结果证实了所测试的三种AMF分离株之间的功能差异，这对于建立潜在的AMF接种剂配方以提高P缺乏土壤中的大豆生产力至关重要。