Abstract

The purpose of this study was to determine the chemical profile of Litsea cubeba essential oil, carry out an in vitro evaluation of its antioxidant potential and its cytotoxicity, as well as its antifungal and antimicotoxigenic activities against Fusarium verticillioides. Most of the compounds observed in the EO were neral (32.75%) and geranial (37.67%). The radical scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid was 104.4 and 56.4 mmol Trolox mg⁻¹, respectively, indicating good antioxidant activity. The EO studied by us revealed cytotoxic effect against HT-29 and HeLa cancer cells. The Minimum Inhibitory and Minimum Fungicidal Concentrations against F. verticillioides were both 125 µg mL⁻¹. Morphological investigation, performed by fluorescence microscopy and scanning electron microscopy, showed that hyphae and microconidia structures underwent changes after treatment with the EO. Analyses performed with the EO strongly reduced the mycelial development of F. verticillioides and the synthesis of fumonisins B₁ and B₂ in dose-dependence effect compared (P < 0.01) with the fungal control (10⁵ conidia mL⁻¹) and positive control (fludioxonil + metalaxyl-M). Thus, the results obtained in vitro suggest that L. cubeba EO has excellent antioxidant, fungicidal, and antimycotoxigenic effects.

摘要

这项研究的目的是确定Litsea cubeba精油的化学特征，对其抗氧化能力和细胞毒性进行体外评估，以及其对镰孢镰刀菌的抗真菌和抗产毒活性。在EO中观察到的大多数化合物是神经性（32.75％）和香叶醛（37.67％）。2,2-二苯基-1-吡啶并肼基和2,2'-叠氮基-双-3-乙基苯并噻唑啉-6-磺酸的自由基清除能力分别为Trolox mg ^-1和56.4 mmol Trolox mg ^-1，表明其良好的抗氧化活性。我们研究的EO显示了对HT-29和HeLa癌细胞的细胞毒性作用。最小的抑制和最小杀真菌浓度拟南芥（F. verticillioides）均为125 µg mL ^-1。通过荧光显微镜和扫描电子显微镜进行的形态学研究表明，在用EO处理后，菌丝和微分生孢子的结构发生了变化。分析与EO进行强烈降低的菌丝发展F.轮枝和伏马菌素B的合成₁和B ₂在剂量依赖性效果相比（P 与真菌控制<0.01）（10 ⁵个分生孢子毫升^-1）和阳性对照（氟二氧嘧啶+甲霜灵-M）。因此，体外获得的结果表明立方体乳杆菌 EO具有出色的抗氧化剂，杀真菌剂和抗霉菌毒素作用。