Overcoming insect immune response: The role of Pseudomonas aeruginosa alkaline protease in phenoloxidase inhibition,Physiological Entomology

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Overcoming insect immune response: The role of Pseudomonas aeruginosa alkaline protease in phenoloxidase inhibition
Physiological Entomology ( IF 1.6 ) Pub Date : 2021-02-28 , DOI: 10.1111/phen.12354
Mariola Andrejko ₁ , Anna Siemińska‐Kuczer ₁ , Bartłomiej Iwański ₁ , Małgorzata Cytryńska ₁

Affiliation

The role of Pseudomonas aeruginosa alkaline protease, one of the important virulence factors of these bacteria, in the prophenoloxidase (proPO) system activation in Galleria mellonella haemolymph was investigated. Immunization of G. mellonella larvae with alkaline protease led to an increase in phenoloxidase (PO) activity in haemolymph 2–8 h after the injection. However, 15 h after the challenge, almost no PO activity was detected, in contrast to insects immunized with heat‐killed P. aeruginosa, where an elevated PO activity in haemolymph persisted at all the time points of the experiment (2–24 h). To test the effect of alkaline protease on already activated PO in vitro, non‐immune G. mellonella haemolymph with proPO system pre‐activated in vitro by heat‐killed P. aeruginosa was used. Subsequently, direct incubation of the protease with non‐immune haemolymph was carried out to test the effect of the alkaline protease on the proPO system activation. It can be postulated that P. aeruginosa alkaline protease affected not only the active PO, but also significantly inhibited proPO cascade activation in the haemolymph of G. mellonella.

中文翻译：

克服昆虫的免疫反应：铜绿假单胞菌碱性蛋白酶在酚氧化酶抑制中的作用

的作用绿脓杆菌碱性蛋白酶，这些细菌的重要毒力因子中的一个，在酶原（酚氧化酶原）激活系统在蜡螟血淋巴进行了研究。的免疫蜡螟幼虫导致注射后在血淋巴2-8 h的增加酚（PO）的活性碱性蛋白酶。但是，在攻击后15小时，几乎没有检测到PO活性，而用热灭活的铜绿假单胞菌免疫的昆虫则在整个实验时间点（2-24 h）持续存在着血淋巴中的PO活性升高。。为了测试碱性蛋白酶对已经激活的体外非免疫G.mellonella PO的影响使用通过热灭活的铜绿假单胞菌在体外预先激活的具有proPO系统的血淋巴。随后，将蛋白酶与非免疫血淋巴直接孵育，以测试碱性蛋白酶对proPO系统激活的影响。可以推测，铜绿假单胞菌碱性蛋白酶不仅影响了活性PO，也显著抑制酚氧化酶原活化级联中的血淋巴蜡螟。

更新日期：2021-05-05

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