Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation,Genes to Cells

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Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation
Genes to Cells ( IF 1.3 ) Pub Date : 2021-02-27 , DOI: 10.1111/gtc.12843
Yukari Okita _{1,

2} , Ling Zheng ₁ , Kunio Kawanishi ₃ , Hirotoshi Miyoshi ₄ , Kazuyoshi Yanagihara ₅ , Mitsuyasu Kato _{1,

2}

Affiliation

Three‐dimensional (3D) culturing mimics the heterogeneous cellular conditions of the in vivo tumor microenvironment compared to 2D monolayer‐cultured cells and 3D cultures of established cancer cell lines (sphere culture) or patient‐derived cancer cells (organoid culture) are frequently used for cancer research or drug screening and evaluation. To establish more cost and time‐efficient 3D culture methods for cancer cell lines, we supplemented sphere culture medium with polyvinyl alcohol (PVA) and found that 3D sphere cultures of breast and pancreatic cancer cell lines were significantly increased. Mechanistically, we found that PVA prevented cell death and promoted cellular proliferation while maintaining levels of stemness‐related gene expression. Furthermore, we showed that polyvinyl formal resin (PVF) 3D scaffolds made by cross‐linked PVA can function in serum‐free, long‐term 3D cultures to support maintenance of sphere‐ or tumor‐like cell masses for diverse cancer cell types. Taken together, we demonstrate the effectiveness of PVA and PVF in human cancer cell line culture protocols.

中文翻译：

聚乙烯醇支架和补充剂可通过减少凋亡和促进细胞增殖来支持人类癌细胞系的3D和球形培养

与2D单层培养的细胞相比，三维（3D）培养可模拟体内肿瘤微环境的异质细胞状况，并且经常使用已建立的癌细胞系（球形培养）或患者来源的癌细胞（有机样培养）的3D培养用于癌症研究或药物筛选和评估。为了建立更多低成本，省时的癌细胞系3D培养方法，我们在球形培养基中添加了聚乙烯醇（PVA），发现乳腺癌和胰腺癌细胞系的3D球形培养显着增加。从机制上讲，我们发现PVA可以防止细胞死亡并促进细胞增殖，同时保持与干性相关的基因表达水平。此外，我们证明了由交联的PVA制成的聚乙烯醇缩甲醛树脂（PVF）3D支架可以在无血清的长期3D培养物中发挥作用，以支持维持各种癌细胞类型的球形或肿瘤样细胞团。两者合计，我们证明了PVA和PVF在人类癌细胞系培养方案中的有效性。

更新日期：2021-02-27

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