The current study evaluated the use of two supplemental microalgae, Spirulina platensis and Tetraselmis chuii, and decapsulated artemia cysts as novel diets for post-weaned common sole (Solea solea) larvae. Four isonitrogenous (55% CP), isoenergetic (22 MJ kg⁻¹) post-weaning diets were prepared; a control, algae-free diet and two control diets supplemented with 50 g kg⁻¹ diet of dried S. platensis (SD) or T. chuii (TD). The fourth diet consisted only of decapsulated artemia cyst powder (AD), enriched with 50 g kg⁻¹ diet of docosahexaenoic acid (DHA). A fifth, commercial marine larval diet was used as a reference diet, for comparison. The diets were fed to S. solea larvae (10 mg fish⁻¹, 42 days post-hatch), at a daily rate of 20% of their live weight, five times a day, for 28 days. The results indicated that AD diet induced the highest growth rates, followed by TD- and SD-supplemented diets, respectively, whereas the control and commercial diets displayed the lowest performance. SD- and AD-fed groups had the highest body protein contents, while body lipids were not significantly affected by dietary treatment, except the AD group which exhibited significantly higher lipid level. The results also indicated that AD- and algae-supplemented diets produced significantly higher survival rate and body n-3 HUFA, EPA, DHA and n-3/n-6 ratio than the control and commercial diets. This study demonstrated that enriched decapsulated artemia cyst powder could be an excellent post-weaning diet for common sole (S. solea) post-larvae. The present results also suggest that inert microdiets enriched with 50 g kg⁻¹ diet of Spirulina platensis or Tetraselmis chuii can serve as excellent diets for these post-larvae.

中文翻译：

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评估添加微藻的日粮和富含脱壳的卤虫孢囊粉作为断奶后普通鳎 (Solea solea) 幼虫的新型日粮

目前的研究评估了使用两种补充微藻，Spirulina platensis和Tetraselmis chuii，以及去壳的卤虫孢囊作为断奶后普通鳎 ( Solea solea ) 幼虫的新型饲料。制备了四种等氮（55% CP）、等能（22 MJ kg ^-1）断奶后日粮；对照、无藻饮食和补充有50 g kg ^-1干燥S. platensis (SD)或T. chuii (TD)的饮食的两种对照饮食。第四种饮食仅由解囊的卤虫孢囊粉 (AD) 组成，富含 50 g kg ^-1二十二碳六烯酸（DHA）饮食。第五种商业海洋幼虫饮食用作参考饮食，以进行比较。将饲料喂给S. solea幼虫（10 毫克鱼^-1，孵化后 42 天），每天按其活重的 20%，每天 5 次，持续 28 天。结果表明，AD 饮食诱导的生长率最高，其次是 TD 和 SD 补充饮食，而对照和商业饮食表现出最低的性能。SD 和 AD 喂养组具有最高的身体蛋白质含量，而身体脂质不受饮食处理的显着影响，除了 AD 组的脂质水平显着更高。结果还表明，与对照和商业饮食相比，添加 AD 和藻类的饮食产生了显着更高的存活率和体内 n-3 HUFA、EPA、DHA 和 n-3/n-6 比率。这项研究表明，富含脱壳的卤虫孢囊粉可能是普通鳎（S. solea) 后幼虫。目前的结果还表明，富含 50 g kg ^-1螺旋藻或Tetraselmis chuii饮食的惰性微量饮食可以作为这些后期幼虫的极好饮食。