The present study reports the surface functionalization of ZnO nanoparticles with functionalized bovine serum albumin (BSA) as a biocompatible system. Sol gel method was adopted for the synthesis of zinc oxide nanoparticles (ZnO NPs). BSA was functionalized with 2-oxo-2H-chromene-3-carboxylic acid (3-CCA) by the DCC (Dicyclohexyl carbodiimide) coupling method. The presence of hydroxyl functional groups in the biopolymer makes it easier to get coupled with carboxylic acid group of the dye. Finally, the ZnO NPs are incorporated into the biomacromolecular system, BSA, which was functionally modified. The various characterizations done for the surface functionalized ZnO NPs are ultraviolet (UV)/visible, Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The antimicrobial resistance of the surface functionalized ZnO NP product were also investigated. The present study also demonstrates the cytotoxicity of this system against Dalton's lymphoma ascites (DLA) cell lines in vitro.

本研究报告功能化的牛血清白蛋白（BSA）作为生物相容性系统的ZnO纳米粒子的表面功能化。采用溶胶凝胶法合成了氧化锌纳米粒子（ZnO NPs）。通过DCC（二环己基碳二亚胺）偶联方法，将BSA用2-oxo-2H-亚甲基-3-羧酸（3-CCA）功能化。生物聚合物中羟基官能团的存在使其更易于与染料的羧酸基团偶联。最后，将ZnO NPs纳入功能上经过修饰的生物大分子系统BSA中。对表面功能化的ZnO NP进行的各种表征是紫外（UV）/可见光，傅立叶变换红外（FTIR），核磁共振（NMR），X射线衍射分析（XRD），扫描电子显微镜（SEM）和透射电子显微镜（TEM）。还研究了表面官能化的ZnO NP产品的抗微生物性。本研究还证明了该系统对道尔顿淋巴瘤腹水（DLA）细胞系的细胞毒性体外。