Carbonated hydroxyapatite (CCaHAp) closely mimics the chemical composition of apatite found in human bone, compared to hydroxyapatite (HA). The present study aimed to prepare CCaHAp nanocomposite using a copolymer of sodium hyaluronate-polyacrylamide (poly(Hya-AAm)) hydrogel as a HA template. Here, a gamma irradiation technique was utilized for preparing poly(Hya-AAm) hydrogel. The hydrogel had a dual function as a HA template and carbon precursor. The CCaHAp nanocomposite was synthesized in situ by impregnating swollen discs of poly(Hya-AAm) hydrogel in the dual solution of phosphoric acid and calcium nitrate. The impregnation cycle was repeated one, two, three, and four times to fabricate four samples of CCaHAp with Ca/P ratios of 1.67, 1.89, 1.48, and 1.37 and carbon content of 8.6, 12.25, 12.24, and 10.07%, respectively, with minor cosubstitution of Na and Mg atoms. Moreover, this study analyzed the effectiveness of CCaHAp compared with HA, which was used as a control for in vitro osteoblast cell regeneration. Four CCaHAp and HA discs were seeded with the MC3T3-E1 osteoblast cells. On day 1, increased cell attachment was observed in the four samples of CCaHAp compared with that in HA discs. Furthermore, compared with the HA control, cell differentiation and regeneration were significantly increased in the CCaHAp samples on day 21, especially in the CCaHAp sample with a Ca/P ratio of 1.89. This study found that the use of CCaHAp as a nanobiocompatible material potentially improved osteogenic ability compared with HA.

与羟基磷灰石（HA）相比，碳酸羟基磷灰石（CCaHAp）与人类骨骼中磷灰石的化学成分非常相似。本研究旨在使用透明质酸钠-聚丙烯酰胺（聚（Hya-AAm））水凝胶的共聚物作为HA模板制备CCaHAp纳米复合材料。在这里，γ辐照技术被用来制备聚（Hya-AAm）水凝胶。水凝胶具有作为HA模板和碳前体的双重功能。CCaHAp纳米复合材料原位合成通过在磷酸和硝酸钙的双重溶液中浸渍聚（Hya-AAm）水凝胶溶胀的椎间盘。重复执行一次，两次，三次和四次浸渍循环，以制作四个CCaHAp样品，Ca / P比分别为1.67、1.89、1.48和1.37，碳含量分别为8.6、12.25、12.24和10.07％，与Na和Mg原子的少量共取代。此外，本研究分析了与用作体外对照的HA相比，CCaHAp的有效性成骨细胞再生。将四个CCaHAp和HA圆盘接种MC3T3-E1成骨细胞。在第1天，与HA椎间盘相比，在四个CCaHAp样品中观察到细胞附着增加。此外，与HA对照相比，第21天CCaHAp样品中的细胞分化和再生显着增加，尤其是在Ca / P比为1.89的CCaHAp样品中。这项研究发现，与HA相比，使用CCaHAp作为纳米生物相容性材料可能会改善成骨能力。