In this work, the incorporation of I4.0 technologies in fermentation was studied. The work aimed to explore if I4.0 technologies could be used to solve problems related to the modernization of fermentation processes, particularly, 1) the interconnection of incompatible components (sensor, compressor, and feeding pump), 2) the implementation of fermentation conditions, relevant to the process efficiency, and 3) making the fermentation an I4.0 compatible component. The technologies were tested on a lab-scale thraustochytrid fermentation, an example of a complex and economically valuable bioprocess. The results showed that the incorporated I4.0 technologies allowed acquiring the dissolved oxygen values from an incompatible equipment screen and implementing a control algorithm for obtaining; high carbon and nitrogen, and low dissolved oxygen concentration automatically after the growth phase. An automatic supervision tool allowed communicating relevant information about the fermentation state to humans and other computers. We conclude that incorporating I4.0 technologies in complex fermentation processes can improve process and equipment integration and allow the implementation of culture conditions that cannot be obtained using I2.0 and I3.0 technologies.

在这项工作中，研究了I4.0技术在发酵中的结合。该工作旨在探讨I4.0技术是否可用于解决与发酵过程现代化相关的问题，特别是1）不兼容组件（传感器，压缩机和进料泵）的互连，2）发酵条件的实现，与过程效率有关，以及3）使发酵成为I4.0兼容组件。这项技术在实验室规模的破囊壶菌发酵中进行了测试，这是一个复杂且具有经济价值的生物过程的例子。结果表明，结合的I4.0技术允许从不兼容的设备屏幕中获取溶解氧值，并实现了用于获取的控制算法。高碳氮 生长阶段后自动降低溶解氧浓度。一个自动监督工具允许将有关发酵状态的相关信息传达给人类和其他计算机。我们得出的结论是，将I4.0技术整合到复杂的发酵过程中可以改善过程和设备集成度，并允许实施使用I2.0和I3.0技术无法获得的培养条件。