Abstract

The dynamics of synthesis and accumulation of protoporphyrin IX induced by 5-aminolevulinic acid (5-ALA) was studied using confocal fluorescence microscopy in three established cell lines: immortalized cells originated from human tumor tissues—epithelial carcinoma (HeLa cells) and lung adenocarcinoma (A549 cells)—and immortalized fibroblast-like mouse embryo cells (3T3 line). The accumulation of protoporphyrin IX in cell cultures was estimated by analyzing the intensity of its fluorescence. The dynamics of changes in the fluorescence intensity with the cell incubation duration in the presence of 5-ALA and with the 5-ALA concentration was studied.

中文翻译：

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5-氨基乙酰丙酸诱导的原卟啉IX在三种不同来源的细胞系中的蓄积动力学

摘要

使用共聚焦荧光显微镜技术在三个已建立的细胞系中研究了由5-氨基乙酰丙酸（5-ALA）诱导的原卟啉IX的合成和积累动力学：源自人类肿瘤组织的永生细胞-上皮癌（HeLa细胞）和肺腺癌A549细胞）和永生化的成纤维细胞样小鼠胚胎细胞（3T3系）。通过分析原卟啉IX的荧光强度来估计原卟啉IX在细胞培养物中的积累。研究了在5-ALA存在下和5-ALA浓度下细胞培养持续时间中荧光强度变化的动力学。