Abstract

The effect of heavy-metal ions (Cd²⁺, Cr⁶⁺, and Cu²⁺) on cyanobacteria sp. Microcystis and sp. Synechocystis has been studied. Since heavy metals impact causes blocking of light-energy transmission channels and arrest of electron transport in photosynthetic apparatus of cyanobacteria, their influence can be detected within a few hours by fluorescence techniques. In this investigation three standard fluorescence techniques are used: fluorescence spectroscopy, pulsed amplitude modulation fluorimetry, and confocal microscopic spectroscopy. All three used methods showed a decrease in photosynthetic activity for cyanobacterial cells exposed to heavy metals. However, the mechanisms of action for different heavy metals are slightly different. In this paper, we analyze variations in spectroscopic and kinetic properties of emmitted fluorescence for cyanobacterial cultures and single living cells in order to identify a specific mechanism of heavy metals influence.

中文翻译：

---

技术性污染物的特定特征对单细胞蓝细菌的光合活性的影响

摘要

重金属离子（Cd 2 ⁺，Cr ⁶⁺和Cu ²⁺）对蓝细菌的影响。微囊藻和sp。结膜囊肿已经研究过了。由于重金属的撞击会导致光能传输通道的阻塞并阻止蓝细菌的光合装置中的电子传输，因此可以通过荧光技术在数小时内检测到它们的影响。在这项研究中，使用了三种标准的荧光技术：荧光光谱法，脉冲振幅调制荧光法和共聚焦显微光谱法。所有这三种使用的方法均显示出暴露于重金属的蓝细菌细胞的光合作用活性降低。但是，不同重金属的作用机理略有不同。在本文中，我们分析了蓝细菌培养物和单个活细胞发出的荧光的光谱和动力学性质的变化，以确定重金属影响的特定机制。