Studies have shown that diesel exhaust particle (DEP), a typical air particulate matter (PM) component of diesel exhaust, exerts its toxic effects by inducing oxidative and pro-inflammatory effects. Naringenin is the predominant flavanone in citrus fruits and has been reported to possess antioxidant, anti-inflammatory, anti-carcinogenic, and anti-mutagenic effects. This study examined the effects of naringenin on the oxidative and inflammatory effects of DEP in aorta and heart of Wistar rats. Diesel exhaust particle collected from a vehicular source in Akure, Nigeria, was extracted in methanol, fractionated and characterized by GCMS. Forty male Wistar rats (80–90 g) were divided into 8 groups. These rats were exposed to either methanol extract of SRM 2975 (mSRM; 0.064 mg/kg) DEP or collected diesel exhaust particles (mDEP; 0.064 or 0.64 mg/kg) sample in the presence or absence of naringenin (50 mg/kg). Both the DEP extracts and naringenin (NAR) were administered by oral gavage. The molecular interactions of NAR with target proteins associated with DEP toxicity were determined using in silico approach. The results show that both mDEP and mSRM caused significant (p < 0.001) decrease in serum HDL but increase serum AST/ALT ratio, ALP, LDL, triacylglycerol (TG), total cholesterol (TC), tissue conjugated dienes (CD) and MDA levels. The extracts also significantly (p < 0.001) increased the expression of IL-1β, TNFα, NF-κB, IκB, and IκKB in the aorta and heart, but decreased Nrf2, HO1, and anti-inflammatory IL-10 mRNA levels when compared with control. Pretreatment with NAR significantly (p < 0.001) reversed all these DEP-induced effects in both tissues. In conclusion, naringenin has the potential to mitigate DEP-induced oxidative stress and inflammation and thus may serve as a therapeutic agent against air pollution-induced cardiovascular damage.

研究表明，柴油机废气颗粒（DEP）是柴油机废气中典型的空气颗粒物（PM）成分，通过诱导氧化和促炎作用发挥其毒性作用。柚皮素是柑橘类水果中的主要黄烷酮，据报道具有抗氧化，抗炎，抗癌和抗诱变作用。这项研究检查了柚皮苷对Wistar大鼠主动脉和心脏中DEP的氧化和炎性作用的影响。从尼日利亚的阿库雷市的汽车来源收集的柴油机废气颗粒用甲醇萃取，分馏并通过GCMS进行表征。将40只雄性Wistar大鼠（80–90 g）分为8组。这些大鼠暴露于SRM 2975（mSRM; 0.064 mg / kg）DEP的甲醇提取物或收集的柴油机排气颗粒（mDEP; 0.064或0）。64 mg / kg）样品中是否存在柚皮素（50 mg / kg）。DEP提取物和柚皮素（NAR）均通过管饲法给药。使用计算机方法确定了NAR与与DEP毒性相关的靶蛋白的分子相互作用。结果表明，mDEP和mSRM均引起了显着（p <0.001）降低血清HDL，但增加血清AST / ALT比，ALP，LDL，三酰甘油（TG），总胆固醇（TC），组织结合二烯（CD）和MDA水平。与提取物相比，提取物还显着（p <0.001）增加了主动脉和心脏中IL-1β，TNFα，NF-κB，IκB和IκKB的表达，但降低了Nrf2，HO1和抗炎性IL-10 mRNA的水平。与控制。用NAR预处理显着（p <0.001）可以逆转这两种组织中所有DEP诱导的作用。总之，柚皮苷具有减轻DEP诱导的氧化应激和炎症的潜力，因此可以作为针对空气污染引起的心血管损害的治疗剂。