This article reports fabrication and characterization of field effect transistor (FET) device consisting of zinc oxide (ZnO) nanoparticle-glutathione-S-transferase (GST) composite channel for successful in vitro detection and quantification of glutathione (GSH) in solution and in cancerous cell. The functionalized channel layer is synthesized by means of electrostatic binding between ZnO nanoparticles and GST. Detection of glutathione can be confirmed by the change in output current characteristics of the device in real time whenever the conjugation reaction between GSH and 1-chloro-2, 4-dinitrobenzene (CDNB) in the presence of GST takes place on the channel of the device. The characterized device had a sensitivity of 60.22 μA/dec and limit of detection (LoD) of 43.96 nM. Thus, from the device characteristics, GSH could be quantified. Subsequently, laboratory grown HeLa, MCF-7 (both cancerous), and human embryonic kidney (HEK-noncancerous) cells were characterized with the fabricated device and both the cancerous cells resulted in significantly higher drain current compared to noncancerous HEK cells. The fabricated device resulted in a sensitivity of 210.86 nA/cell and LoD of 30 cells when characterized with HeLa cells. This detection paves way for a device-based early detection scheme for cancer by measuring the GSH concentration in human cell cytosol.

中文翻译：

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用于检测谷胱甘肽的谷胱甘肽-S-转移酶-nO纳米共轭整体 FET 器件的制造


本文报道了由氧化锌 (ZnO) 纳米颗粒-谷胱甘肽-S-转移酶 (GST) 复合通道组成的场效应晶体管 (FET) 器件的制造和表征，用于成功体外检测和定量溶液中和癌组织中的谷胱甘肽 (GSH)细胞。功能化通道层是通过 ZnO 纳米颗粒和 GST 之间的静电结合合成的。每当 GST 存在下，GSH 与 1-氯-2, 4-二硝基苯 (CDNB) 发生缀合反应时，器件的通道上发生共轭反应，即可通过器件输出电流特性的实时变化来确认谷胱甘肽的检测。设备。表征装置的灵敏度为 60.22 μA/dec，检测限 (LoD) 为 43.96 nM。因此，根据器件特性，GSH 可以被量化。随后，使用所制造的装置对实验室培养的 HeLa、MCF-7（均为癌性）和人胚肾（HEK 非癌性）细胞进行了表征，与非癌性 HEK 细胞相比，这两种癌性细胞均产生显着更高的漏极电流。当使用 HeLa 细胞进行表征时，所制造的装置的灵敏度为 210.86 nA/细胞，LoD 为 30 个细胞。该检测通过测量人体细胞胞质中的 GSH 浓度，为基于设备的癌症早期检测方案铺平了道路。