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Two-color differential dynamic microscopy for capturing fast dynamics
Review of Scientific Instruments ( IF 1.3 ) Pub Date : 2021-02-10 , DOI: 10.1063/5.0039177
Ruilin You 1 , Ryan McGorty 1
Affiliation  

Differential dynamic microscopy (DDM) is increasingly used in the fields of soft matter physics and biophysics to extract the dynamics of microscopic objects across a range of wavevectors by optical microscopy. Standard DDM is limited to detecting dynamics no faster than the camera frame rate. We report on an extension to DDM where we sequentially illuminate the sample with spectrally distinct light and image with a color camera. By pulsing blue and then red light separated by a lag time much smaller than the camera’s exposure time, we are able to use this two-color DDM method to measure dynamics occurring much faster than the camera frame rate.

中文翻译:

两色差分动态显微镜,可捕捉快速动态

差分动态显微镜(DDM)越来越多地用于软物质物理学和生物物理学领域,以通过光学显微镜在一系列波矢上提取微观物体的动力学。标准DDM限于仅以不超过摄像机帧频的速度检测动态。我们报告了DDM的扩展,在DDM中我们依次用光谱不同的光和彩色相机照亮样品。通过脉冲蓝光和红光,使它们之间的间隔时间比相机的曝光时间小得多,我们可以使用这种双色DDM方法来测量比相机帧速率快得多的动态。
更新日期:2021-02-26
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