Nanotechnology deals with the synthesis of materials and particles at nanoscale with dimensions of 1–100 nm. Biological synthesis of nanoparticles, using microbes and plants, is the most proficient method in terms of ease of handling and reliability. Core objectives of this study were to synthesize metallic nanoparticles using selenium metal salt from citrus fruit extracts, their characterization and evaluation for antimicrobial activities against pathogenic microbes. In methodology, simple green method was implicated using sodium selenite salt solution and citrus fruit extracts of Grapefruit and Lemon as precursors for synthesizing nanoparticles. Brick red color of the solution indicated towards the synthesis of selenium nanoparticles (SeNPs). Nanoparticle’s initial characterization was done by UV–Vis Spectrophotometry and later FTIR analysis and DLS graphs via Zetasizer were obtained for the confirmation of different physical and chemical parameters of the nanoparticles. Different concentrations of SeNPs were used for antimicrobial testing against E. coli, M. luteus, B. subtilis and K. pneumoniae comparative with the standard antibiotic Ciprofloxacin. SeNPs possessed significant antimicrobial activities against all the bacterial pathogens used. Conclusively, SeNPs made from citrus fruits can act as potent antibacterial candidates.

纳米技术涉及尺寸为 1-100 nm 的纳米级材料和粒子的合成。就易于处理和可靠性而言，使用微生物和植物生物合成纳米颗粒是最熟练的方法。本研究的核心目标是使用来自柑橘类水果提取物的硒金属盐合成金属纳米颗粒，对其进行表征和评估对病原微生物的抗菌活性。在方法学上，简单的绿色方法涉及使用亚硒酸钠盐溶液和葡萄柚和柠檬的柑橘类水果提取物作为合成纳米颗粒的前体。溶液的砖红色表明硒纳米颗粒 (SeNPs) 的合成。纳米粒子的初始表征是通过紫外-可见分光光度法完成的，随后通过 Zetasizer 进行 FTIR 分析和 DLS 图，以确认纳米粒子的不同物理和化学参数。不同浓度的 SeNP 用于抗微生物测试大肠杆菌、藤黄分枝杆菌、枯草芽孢杆菌和肺炎克雷伯菌与标准抗生素环丙沙星比较。SeNPs 对所有使用的细菌病原体都具有显着的抗菌活性。最后，由柑橘类水果制成的 SeNPs 可以作为有效的抗菌候选物。