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An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
Scientific Reports ( IF 3.8 ) Pub Date : 2021-02-26 , DOI: 10.1038/s41598-021-84237-2
Kentaro Masujin 1 , Tomoya Kitamura 1 , Ken -Ichiro Kameyama 1 , Kota Okadera 1 , Tatsuya Nishi 2 , Takato Takenouchi 3 , Hiroshi Kitani 3 , Takehiro Kokuho 1
Affiliation  

African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a fatal hemorrhagic disease of domestic pigs and wild boar. The virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainable ASFV-sensitive cell lines, but which supported only low viral replication levels of limited, mostly artificially attenuated strains of ASFV. Here, we examined the competence of a novel cell line of immortalized porcine kidney macrophages (IPKM) for ASFV infection. We demonstrated that IPKM cells can facilitate high levels (> 107.0 TCID50/mL) of viral replication of ASFV, and hemadsorption reactions and cytopathic effects were observed as with porcine alveolar macrophages when inoculated with virulent field isolates: Armenia07, Kenya05/Tk-1, and Espana75. These results suggested that IPKM may be a valuable tool for the isolation, replication, and genetic manipulation of ASFV in both basic and applied ASF research.



中文翻译:

一种能够分离非洲猪瘟病毒的永生化猪巨噬细胞系

非洲猪瘟病毒 (ASFV) 是非洲猪瘟 (ASF) 的病原体,ASF 是家猪和野猪的一种致命的出血性疾病。该病毒主要感染巨噬细胞和单核细胞宿主细胞,这些细胞不会在体外生长。已经进行了许多尝试来建立可持续的 ASFV 敏感细胞系,但仅支持有限的、主要是人工减毒的 ASFV 株的低病毒复制水平。在这里,我们检查了一种新型永生化猪肾巨噬细胞 (IPKM) 细胞系对 ASFV 感染的能力。我们证明了 IPKM 细胞可以促进高水平 (> 10 7.0 TCID 50/mL) 的 ASFV 病毒复制,观察到与猪肺泡巨噬细胞一样,当接种有强毒力的野战分离株:Armenia07、Kenya05/Tk-1 和 Espana75 时发生血细胞吸附反应和细胞病变效应。这些结果表明,在基础和应用 ASF 研究中,IPKM 可能是 ASFV 分离、复制和遗传操作的宝贵工具。

更新日期:2021-02-26
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