Abstract

Purpose

Under natural conditions, the reaction of living organisms to the action of acute gamma radiation depends on other stressors, including heavy metals. The aim of this work was to study changes in morphometric parameters, the content of photoassimilation pigments and the level of oxidative stress in irradiated duckweed at various copper concentrations in the culture medium.

Materials and methods

As a model organism, we used Lemna minor L. Duckweed was exposed to acute γ-radiation at doses of 18, 42, 63 Gy. After irradiation, the plants were transferred into a medium containing 3, 5, 6.3 μmol/L Cu. On the 4th day of exposure, the levels of chlorophyll, carotenoids, malondialdehyde (MDA) were measured; after 7 days, the specific growth rate, the level of damage, the change in the frond area, copper concentration in plant tissues were determined.

Results

The action of γ-radiation (18, 42, 63 Gy) and copper ions (3, 5, 6.3 μmol/L) reduced the growth rate, increased the membrane lipid peroxidation, reduced the area of the fronds more significantly than under the separate action of the factors. The factors acted antagonistically on the specific growth rate. The content of copper in the tissues of irradiated plants (42, 63 Gy) increased.

Conclusion

Irradiation of duckweed with acute doses of gamma radiation reduced the resistance of plants to excess copper in the environment.

中文翻译：

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伽马辐射和暴露于铜离子对 Lemna minor L 的联合作用

摘要

目的

在自然条件下，活生物体对急性伽马辐射作用的反应取决于其他压力源，包括重金属。这项工作的目的是研究培养基中不同铜浓度下辐照浮萍的形态学参数、光同化色素含量和氧化应激水平的变化。

材料和方法

作为模型生物，我们使用小浮萍 L. Duckweed 暴露于 18、42、63  Gy 剂量的急性 γ 辐射。照射后，将植物转移到含有 3、5、6.3 μmol/L Cu 的培养基中。暴露第4天，测定叶绿素、类胡萝卜素、丙二醛（MDA）水平；7天后，测定比生长率、损伤程度、叶面积变化、植物组织中铜浓度。 

结果

γ-辐射（18, 42, 63 Gy）和铜离子（3, 5, 6.3  μ mol/L）的作用降低了生长速率，增加了膜脂过氧化，减少了叶面积比下更显着。因素的单独作用。这些因素对特定增长率起反作用。辐照植物（42, 63 Gy）组织中铜的含量增加。

结论

用急性剂量的伽马射线辐照浮萍会降低植物对环境中过量铜的抵抗力。