ABSTRACT

The present study investigated the effect of arbuscular mycorrhizal fungus (AMF) Rhizophagus intraradices on plant growth and phosphorus (P) uptake of soybean (Glycine max L.) in soils contaminated with different concentrations of heavy metals [copper (100 and 300 mg kg⁻¹), lead (100 and 300 mg kg⁻¹) and zinc (300 and 600 mg kg⁻¹)] and combination (Cu100 + Pb100 + Zn300) in a pot experiment. The bioaccumulation characteristics of the heavy metals in the plant tissues in terms of bioabsorption coefficient (BAC), bioconcentration factor (BCF) and translocation factor (TF) were also evaluated. The results showed R. intraradices inoculation significantly (p < .01) enhanced soybean growth, P uptake and grain yield in all polluted soils compared to non-inoculated treatment. In addition, R. intraradices retained most of the heavy metals in the roots, with lesser translocation to the shoots and seeds. Exposure to 600 mg Zn kg⁻¹ soil remarkably reduced the plant growth, P content and seed yield of the soybean. Copper uptake in the shoot was significantly increased in multi-metal contaminated soil with reduced lead and zinc compared to single polluted soils. This work suggests that Rhizophagus intraradices inoculation may be a potential and effective of AMF isolate for enhancing plant growth, P nutrition and stress tolerance in heavy metal-contaminated soil

摘要

本研究调查了丛枝菌根真菌 (AMF) Rhizophagus intraradices对植物生长和大豆 ( Glycine max L.) 在被不同浓度重金属污染的土壤中磷 (P) 吸收的影响[铜 (100 和 300 mg kg ^{- 1} )、铅（100 和 300 mg kg ^-1）和锌（300 和 600 mg kg ^-1）] 和组合（Cu100 + Pb100 + Zn300）在盆栽实验中。还评估了植物组织中重金属在生物吸收系数（BAC）、生物富集因子（BCF）和易位因子（TF）方面的生物积累特性。结果显示R.intraradices接种显着（p< .01) 与未接种处理相比，所有受污染土壤中的大豆生长、磷吸收和谷物产量均有所提高。此外，R. intraradices保留了根中的大部分重金属，向芽和种子的转移较少。暴露于 600 mg Zn kg ^-1土壤显着降低了大豆的植物生长、P 含量和种子产量。与单一污染土壤相比，在铅和锌减少的多金属污染土壤中，枝条对铜的吸收显着增加。这项工作表明，Rhizophagus intraradices接种可能是 AMF 分离物在重金属污染土壤中增强植物生长、磷营养和胁迫耐受性的一种潜力和有效的方法。