ABSTRACT

We designed and synthesised a novel molecular probe exhibiting high selectivity and sensitivity towards reactive sulphur species (RSS) over other amino acids and biologically relevant species, as well as scrutinised its spectroscopic behaviours under physiological conditions and in living milieu. We used an electrophilic cyanate group as a masking agent to block the excited state intramolecular proton transfer process of 2-(2-cyanato-3-methoxyphenyl)benzo[d]thiazole (HMBT-OCN), which readily hydrolyses to the highly fluorescent structure, 2-(2ʹ-Hydroxy-3ʹ-methoxyphenyl)benzothiazole (HMBT-OH), in the presence of reactive sulphur species.

摘要

我们设计并合成了一种新型分子探针，该探针对其他氨基酸和生物学相关物种具有很高的选择性和对活性硫物种（RSS）的敏感性，并仔细研究了其在生理条件下和生活环境中的光谱行为。我们使用亲电子的氰酸酯基作为掩蔽剂来阻止2-（2-氰基-3-甲氧基苯基）苯并[d]噻唑（HMBT-OCN）的激发态分子内质子转移过程，该过程易于水解为高荧光结构，在反应性硫原子的存在下，生成2-（2ʹ-羟基-3ʹ-甲氧基苯基）苯并噻唑（HMBT-OH）。