ABSTRACT

In the present study, we assessed the agronomic value of a formulated plant growth-promoting rhizobacteria (PGPR) consortium combined with a phosphocompost amendment in deficient soil, using three plant models (Phaseolus vulgaris, Zea mays, and Solanum lycopersicum). PGPR consortium contains four strains which have been identified as Pseudomonas sp. DN 13–01, Sphingobacterium suaedae T47, Bacillus pimilus X22, and Bacillus cereus 263AG5, respectively. They have previously displayed their P solubilizing capacity and to have PGPR traits. In this study, the four strains were able also to dissolve potassium (K), with K solubility (KS) indices range between 0.950.03 and 1.210.25 in solid medium and released K values between 30.251.21 and 45.230.84 g-L⁻¹in liquid medium. The bacterial strains also produced acid and alkaline phosphatases, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, protease, cellulase, pectinase, lipase and amylase enzymes. The amendment of deficient soil with phosphocompost enriched with the PGPR consortium increased much more soil productivity and plant growth compared with controls uninoculated. The formulated PGPR consortium combined with phosphocompost gave the best yield in terms of plant height, number of leaves, number of fruits, shoot biomass, root biomass, and dry shoot biomass of the three-plant studied. Simultaneously, the PGPR consortium inoculation increased P and K uptake in the shoots and available P and K in the soil. In summary, our results showed that the application of PGPR consortium combined with phosphocompost amendment increased plant growth in deficient soil.

摘要

在本研究中，我们使用三种植物模型（菜豆（Phaseolus vulgaris），玉米（Zea mays）和茄茄（Solanum lycopersicum））评估了在贫瘠土壤中配成植物生长的根际细菌（PGPR）组合与磷酸堆肥组合的农艺价值。PGPR财团包含四个被鉴定为Pseudomonas sp的菌株。DN 13–01，suaedae suaedae T47，短杆菌芽孢杆菌X22和蜡状芽孢杆菌分别为263AG5。他们以前表现出了对磷的增溶能力，并具有PGPR性状。在这项研究中，四个菌株能够也溶解钾（K），其中K溶解度（KS）指数范围30.251.21 45.230.84和GL之间在固体培养基0.950.03和1.210.25和释放的K值之间^{- 1个}在液体介质中。细菌菌株还产生酸性和碱性磷酸酶，1-氨基环丙烷-1-羧酸酯（ACC）脱氨酶，蛋白酶，纤维素酶，果胶酶，脂肪酶和淀粉酶。与未接种对照相比，用富含PGPR财团的磷酸堆肥对土壤不足的改良增加了土壤生产力和植物生长。配制的PGPR财团与磷酸堆肥组合后，在所研究的三株植物的株高，叶片数，果实数，枝生物量，根生物量和干枝生物量方面，产量最高。同时，PGPR联盟接种增加了芽中P和K的吸收以及土壤中有效P和K的吸收。总之，