Palm oil mill effluent (POME) is a palm industrial waste by‐product that is readily available and possesses high quantities of the organic compounds that are necessary for microbial growth. This study investigated the potential of immobilized Aspergillus oryzae whole cells expressing Candida antartica lipase B (r‐CALB) when using a POME‐based medium as a low‐cost carbon source within biomass support particles. The dry cell weight, hydrolytic activity, and remaining protein were measured for a variety of POME (0–2% w/v) and carbon source concentrations (0–2% w/v) with an incubation time of 96 h. Using POME in the culture medium as a carbon source instead of glucose doubled the retention of cell weight from 1.16 ± 0.03 to 2.36 ± 0.02 g within the biomass support particles and improved the hydrolytic activity from 1.61 ± 0.02 to 2.23 ± 0.02 U mg⁻¹ in whole‐cell lipase. This immobilized r‐CALB was used as a catalyst to esterify POME into biodiesel. This synthesis produced an ester content of 97.52 ± 0.21% w/w after 96 h. Immobilized r‐CALB was reusable for more than ten batches, and resulted in an ethyl ester content of more than 90% w/w even after 10 cycles. The present study demonstrated that POME could serve as a sustainable carbon source for industrial microbial culture with the additional incentive of being a treatment that is inexpensive and sustainable. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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利用棕榈油磨坊废水（POME）固定米曲霉全细胞脂肪酶菌株用于生物柴油合成

棕榈油厂的废液（POME）是一种棕榈工业废品，很容易获得，并且含有微生物生长所必需的大量有机化合物。这项研究调查了表达南极假丝酵母的固定化米曲霉全细胞的潜力脂肪酶B（r-CALB），当使用基于POME的介质作为生物质载体颗粒中的低成本碳源时。在96小时的孵育时间下，测量了各种POME（0–2％w / v）和碳源浓度（0–2％w / v）的干细胞重量，水解活性和剩余蛋白质。在培养基中使用POME代替碳作为碳源，生物质载体颗粒中的细胞重量保留量从1.16±0.03倍增加到2.36±0.02 g，水解活性从1.61±0.02改善到2.23±0.02 U mg ^-1在全细胞脂肪酶中 这种固定化的r-CALB用作将POME酯化为生物柴油的催化剂。96小时后，该合成产生的酯含量为97.52±0.21％w / w。固定化的r-CALB可以重复使用超过十批，即使经过10个循环，其乙酯含量仍超过90％w / w。本研究表明，POME可以作为工业微生物培养的可持续碳源，并且具有诱因，使其成为一种廉价且可持续的治疗方法。©2021化学工业协会和John Wiley＆Sons，Ltd