Triclosan (TCS) and methyl-triclosan (MTCS), an environmental transformation product of biocide of TCS, have been detected in water, sediment, fish, and invertebrates. In this study, the key pathway perturbation in zebrafish (Danio rerio) embryos exposed to TCS (300 μg/L) and TCS/MTCS mixture (300 μg/L TCS + 30 μg/L MTCS) was assessed by integrating the metabolomic and transcriptomic dysregulation. The differential expressed genes (DEGs) were obtained from the subtracted cDNA libraries by using the suppression subtractive hybridization and next-generation sequencing approach. The dysregulation of twenty-eight GO terms and four KEGG pathways, including oxidative phosphorylation and cardiac muscle contraction, were shown in the TCS treatment group, indicating that TCS could disrupt the mitochondrial inner membrane function by downshifting the electrochemical gradient. Meanwhile, the addition of MTCS in the exposure would cause fourteen additional significant KEGG pathway changes, demonstrating the different effects between two exposure. A pathway-based analysis using the identified DEGs and the altered metabolites in zebrafish embryos treated with TCS and TCS/MTCS mixture, collectively, has been applied. This study demonstrated that the integration of SSH-NGS and metabolomics could reveal toxic effects and potential diseases associated with the exposures of TCS and MTCS in aquatic environments.

已在水，沉积物，鱼类和无脊椎动物中检测到三氯生（TCS）和甲基三氯生（MTCS）（TCS杀生物剂的环境转化产物）。在这项研究中，斑马鱼的关键途径摄动（Danio rerio）通过整合代谢组和转录组失调来评估暴露于TCS（300μg/ L）和TCS / MTCS混合物（300μg/ L TCS + 30μg/ L MTCS）的胚胎。通过使用抑制性消减杂交和下一代测序方法，从扣除的cDNA文库中获得差异表达基因（DEG）。TCS治疗组显示28个GO词和4个KEGG通路失调，包括氧化磷酸化和心肌收缩，表明TCS可以通过降低电化学梯度来破坏线粒体内膜功能。同时，在暴露中添加MTCS将导致另外十四次显着的KEGG途径变化，表明两次暴露之间的不同影响。基于已鉴定的DEG和经TCS和TCS / MTCS混合物处理的斑马鱼胚胎中代谢物的改变，已进行了基于途径的分析。这项研究表明，SSH-NGS与代谢组学的整合可以揭示在水生环境中与TCS和MTCS暴露相关的毒性作用和潜在疾病。