In this study, semi-starvation fluctuation driving PD granules cultivation in situ by microorganism exudate metabolites feedbacks was firstly investigated. The PD granules of high nitrite production were cultivated with an excellent mean nitrate-to-nitrite transformation rate (NTR) of 56.39% in just 30 days. The granules size was improved from the initial size of 0.09 ± 0.01 mm in diameter to a size above 2 mm when the extracellular polymeric substance (EPS) content increased from 80.21 ± 10.20 mg/g MLVSS to 777.00 ± 22.13 mg/g MLVSS. Acyl-homoserine lactone signals (AHLs) ultimately increased ten-fold more than the initially through 30 days of cultivation. Meanwhile, Thauera had been identified as the main function bacteria of PD, which enriched from 0.47% to 10.67%. Results demonstrated that AHLs, EPS, PD bacteria and the PD granules cultivation were closely associated. Semi-starvation fluctuation produced oligotrophic stress on bacterial community, a part of bacteria would be eliminated on starvation for oligotrophic stress and AHLs of bacteria regarded as distress signals resulted in the rapid formation of PD granules. A mechanism for PD granular cultivation with semi-starvation fluctuation was proposed from the aspect of oligotrophic stress. A better strategy for rapid PD granules cultivation was obtained and it could be useful for the mainstream granule-based PD combined with the anammox process application in the future.

在这项研究中，首先研究了半饥饿波动驱动微生物分泌物代谢产物反馈原位培养PD颗粒。在短短30天内，以高的平均硝酸盐转化为亚硝酸盐转化率（NTR）达到56.39％的条件，培养了高亚硝酸盐产量的PD颗粒。当细胞外聚合物（EPS）含量从80.21±10.20 mg / g MLVSS增加到777.00±22.13 mg / g MLVSS时，颗粒尺寸从初始直径0.09±0.01 mm改善到2 mm以上。到开始培养30天，酰基高丝氨酸内酯信号（AHL）最终比最初增加了十倍。同时，Thauera被鉴定为PD的主要功能细菌，其含量从0.47％增至10.67％。结果表明，AHL，EPS，PD细菌和PD颗粒的培养密切相关。半饥饿波动对细菌群落产生寡营养胁迫，饥饿时将消除一部分细菌的寡营养胁迫，并且细菌的AHL被认为是遇险信号，导致PD颗粒的快速形成。从贫营养胁迫的角度出发，提出了一种半饥饿波动的PD颗粒培养机理。获得了一种更好的快速PD颗粒培养策略，并且该方法对于将来基于主流颗粒的PD结合厌氧氨氧化工艺的应用可能是有用的。