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Characterization of difference in muscle volatile compounds between triploid and diploid crucian carp
Aquaculture Reports ( IF 3.2 ) Pub Date : 2021-02-25 , DOI: 10.1016/j.aqrep.2021.100641
Li Cai , Zhipeng Ao , Tao Tang , Fangle Tong , Zehong Wei , Fuzhong Yang , Yuqing Shu , Shaojun Liu , Kangsen Mai

The present study was conducted to investigate the volatile compounds profile of raw muscle between diploid (2n = 100) and triploid crucian carp (produced by hybridization from mating diploid crucian carp to allotetraploid, 3n = 150) cultured under the same conditions. Volatile compounds of crucian carp between ploidy were qualitatively analyzed by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry (HS-SPME-GC/MS). A total of 52 volatiles were identified in the two groups of crucian carp including 5 alcohols, 5 aromatics, 8 aldehydes, 1 ketone, 10 alkanes, 19 alkenes and 4 esters. No significant difference was observed in the total relative concentration between the two groups (P > 0.05). Based on the data of odor-active value (OAV ≥ 1), 10 odor-active compounds may mainly contribute to the overall odor, including 1-nonanol, 1-octen-3-ol, hexanal, decanal, octanal, 1-nonanal, p-cymene, styrene, limonene and tridecane. Triploid crucian carp had more odor-active compounds and higher total OAV value than that of diploid (P < 0.05). With the results of partial least squares discrimination analysis (PLS-DA) in total volatiles and odor-active compounds, the overall odor of triploid and diploid crucian carp was obviously different.



中文翻译:

三倍体和二倍体cru鱼肌肉挥发性化合物差异的表征

本研究旨在研究在相同条件下培养的二倍体(2n = 100)和三倍体cru鱼(通过交配二倍体cru鱼与异源四倍体的杂交产生的3n = 150)之间生肌肉的挥发性化合物分布。通过顶空固相微萃取和气相色谱-串联质谱(HS-SPME-GC / MS)定性分析了loid鱼之间的挥发性化合物。在两组of鱼中总共鉴定出52种挥发物,包括5种醇,5种芳烃,8种醛,1种酮,10种烷烃,19种烯烃和4种酯。两组之间的总相对浓度无显着性差异(P> 0.05))。根据气味活性值(OAV≥1)的数据,可能有10种气味活性化合物主要构成总体气味,包括1-壬醇,1-辛烯-3-醇,己醛,癸醛,辛酸,1-壬醛。 ,对-甲基异丙基苯,苯乙烯,柠檬烯和十三烷。三倍体cru鱼比二倍体具有更多的气味活性化合物和更高的总OAV值(P <0.05)。根据总挥发物和气味活性化合物的偏最小二乘判别分析(PLS-DA)结果,三倍体and鱼和二倍体cru鱼的总气味明显不同。

更新日期:2021-02-26
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