Loggerhead sea turtle (C. caretta) is the official European bioindicator of marine litter in the Mediterranean Sea. In 2019, 8 sea turtles, out of 28 specimens loggerhead on the Adriatic coast of Molise, were subjected to necropsy. The intestinal contents were collected and the microplastics until 0.45 μm were extracted. Qualitative and quantitative assessments were performed by stereomicroscope observation and spectroscopic analyses (attenuated total reflection-Fourier transform infrared spectroscopy, ATR-FTIR and Raman microspectroscopy, RMS). Moreover, the analytical quantification of polyethylene terephthalate (PET), polycarbonate (PC), para phthalic acid (PTA) and bisphenol A (BPA) in fat and liver tissues was performed by LC-MS/MS. Microparticles ranging from 0.45 μm to 1 mm were found in all turtles, for a total of 623, while plastic litter greater than 1 mm were found only in 4 specimens (ranging from 0.03 to 0.11 g). Nineteen different polymers and 10 pigments, including polyester (100% of animals), high-density polyethylene (50%) and polypropylene (50%) were identified. BPA, PTA and PET were detected in fat and liver tissues of all animals, while PC was found only in 50%. A major prevalence was registered in the abdominal fat tissue, although only PC compounds were significantly higher in abdominal tissue (p < 0.05), except for free PTA with liver tissue being the most contaminated (p < 0.05). Microplastics and additives surely impact the health status of turtles that showed gastrointestinal impairment and an important level of contamination in tissues.

Graphical abstract

中文翻译：

---

微塑料和添加剂对在中亚得里亚海沿岸搁浅的Log的健康状况的潜在影响

ger海龟（C. etta）是地中海海洋垃圾的官方欧洲生物指标。2019年，对莫利塞亚得里亚海沿岸28个标头的8只海龟进行了尸检。收集肠内容物并提取微塑料直至0.45μm。通过立体显微镜观察和光谱分析（衰减全反射-傅立叶变换红外光谱法，ATR-FTIR和拉曼显微光谱法，RMS）进行定性和定量评估。此外，通过LC-MS / MS对脂肪和肝组织中的聚对苯二甲酸乙二醇酯（PET），聚碳酸酯（PC），对苯二甲酸（PTA）和双酚A（BPA）进行了定量分析。在所有海龟中发现的微粒范围为0.45μm至1 mm，共有623个，而仅在4个样本中发现了大于1毫米的塑料垃圾（范围从0.03到0.11 g）。确定了19种不同的聚合物和10种颜料，包括聚酯（占动物的100％），高密度聚乙烯（占50％）和聚丙烯（占50％）。在所有动物的脂肪和肝组织中均检测到BPA，PTA和PET，而PC中仅检测到50％。尽管腹部脂肪组织中仅PC化合物含量明显较高，但腹部脂肪组织中的流行率最高（p  <0.05），但游离PTA的肝组织污染最严重（p  <0.05）。微量塑料和添加剂无疑会影响龟的健康状况，这些龟表现出肠胃功能障碍和组织中重要的污染水平。

图形概要