Teosinte Branched, Cycloidea, Proliferating Cell Factor proteins are a large family of transcription factors in angiosperms and are specific for each plant species, presenting a highly conserved TCP domain. In plants, they play a key role in the development and metabolism processes. In this study MaTCP genes have been updated, so, 46 MaTCP, 12 MbTCP and 25 MiTCP genes were identified in Musa acuminata, Musa balbisiana and Musa itinerans genomes, respectively. Chromosome location, gene structure, sequence alignment, gene classification and conserved domains of MaTCP, MbTCP and MiTCP proteins were predicted and analyzed. Our phylogenetic analysis classified MaTCP, MbTCP and MiTCP genes into two classes, which was further supported by the conserved motif structure and exon-intron organization. Inquiry in the promoter region suggested the possible roles of TCP banana genes in biological and physiological processes. RNA-seq analysis indicated MiTCP3-MiTCP5, MiTCP9, MiTCP20, MiTCP21, MiTCP33, MiTCP41 and MITCP44 took part during wild banana responsiveness at both normal and low temperatures growing. Gene evolution indicated a high conservation level within monocots TCP genes through orthologous identification between banana, rice, maize and Arabidopsis. Moreover, similar functionality and a high level of conservation were also seen through homology relationship analysis between MiTCP and MbTCP genes with MaTCP genes. The present study provides valuable information about characterization, evolution, diversity and functionality of MaTCP, MbTCP and MiTCP genes and will contribute to better understanding of the biological and physiological roles of TCP-TF in plants.

Teosinte分支，摆线，增殖细胞因子蛋白是被子植物中转录因子的一大家族，对每种植物都具有特异性，具有高度保守的TCP结构域。在植物中，它们在发育和代谢过程中起关键作用。在这项研究中，已经更新了MaTCP基因，因此，在尖头穆萨（Mus acuminata），巴尔比西亚香蕉（Musa balbisiana）和伊塔木兰（Musa itinerans）基因组中分别鉴定出46个MaTCP，12个MbTCP和25个MiTCP基因。预测和分析了MaTCP，MbTCP和MiTCP蛋白质的染色体位置，基因结构，序列比对，基因分类和保守域。我们的系统发育分析分类MaTCP，MbTCP和MiTCP基因分为两类，这由保守的基序结构和外显子-内含子组织进一步支持。在启动子区域的查询表明TCP香蕉基因在生物和生理过程中的可能作用。RNA-seq分析表明，MiTCP3-MiTCP5，MiTCP9，MiTCP20，MiTCP21，MiTCP33，MiTCP41和MITCP44在正常和低温生长过程中均参与了野生香蕉的响应。基因进化表明，通过对香蕉，水稻，玉米和拟南芥的直系同源鉴定，单子叶植物TCP基因内的保护水平很高。。此外，通过MiTCP和MbTCP基因与MaTCP基因之间的同源性关系分析，也可以看到相似的功能和高度保守性。本研究提供了有关MaTCP，MbTCP和MiTCP基因的表征，进化，多样性和功能的有价值的信息，并将有助于更好地理解TCP-TF在植物中的生物学和生理作用。