Abstract—

Soil microbes exert a critical effect on the sustainability of urban lakeside wetland ecosystem. Yet, the magnitude and direction to which ecological restoration affects the soil microbial composition and function remain unclear. Thus, this study was carried out to investigate the structural and functional alterations of soil bacterial community under different restoration patterns, including abandoned shoaly grassland (GL) to cultivated flower land (FL), reed shoaly land (RL) and poplar plantation land (PL). 16S rRNA genes sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were used to determine the genetic diversity and functions of soil bacteria. The results demonstrated that the diversity and richness of soil bacterial community in GL were significantly reduced compared to the other three patterns (P < 0.05). The Proteobacteria phylum, candidatus Saccharibacteria and Gemmatimonas and Sphingomonas genera were the dominant groups in GL pattern, as different from Proteobacteria, Acidobacteria and Gp6 in RL, PL and FL patterns. PICRUSt analysis revealed that the relative frequencies of 26 pathways in Level 2 and 144 pathways in Level 3 were significantly higher in RL, FL and PL patterns than in GL pattern (corrected q value < 0.05). After fifteen years of recovery, natural and artificial restoration increased the diversity of bacterial communities and enrichment in soil nutrients. Some functional genes involved in membrane transport, replication and repair and energy metabolism remarkably promoted the nutrient cycle and metabolic activities in oligotrophic environment (GL), while their relative frequencies could be decreased with the improvement of soil microhabitat during ecological restoration.

中文翻译：

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华东巢湖湖滨湿地恢复十五年后土壤细菌群落组成的结构和功能变化

摘要—

土壤微生物对城市湖滨湿地生态系统的可持续性具有至关重要的作用。然而，生态恢复影响土壤微生物组成和功能的程度和方向仍不清楚。因此，本研究旨在调查不同恢复模式下土壤细菌群落的结构和功能变化，包括废弃的浅滩草地（GL）到耕种的花地（FL），芦苇浅滩地（RL）和杨树人工林地（PL） ）。通过对16S rRNA基因的测序和对群落的系统发育研究（PICRUSt）来确定土壤细菌的遗传多样性和功能。P <0.05）。的变形菌门，暂定Saccharibacteria和Gemmatimonas和鞘氨醇单属均GL图案占主导地位的基团，如在RL，PL和FL图案从变形菌，酸杆菌门和GP6不同。PICRUSt分析显示，RL，FL和PL模式中2级26条路径和3级144条路径的相对频率显着高于GL模式（校正后的q值<0.05）。经过15年的恢复，自然和人工恢复增加了细菌群落的多样性和土壤养分的富集。某些参与膜运输，复制和修复以及能量代谢的功能基因显着促进了贫营养环境（GL）的养分循环和代谢活动，但随着生态恢复过程中土壤微生境的改善，它们的相对频率可能会降低。