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Preparation and properties of fluorescent quantum dots microbeads encapsulated in-situ by polyisobornyl methacrylate for immunochromatography
Journal of Nanophotonics ( IF 1.5 ) Pub Date : 2021-02-01 , DOI: 10.1117/1.jnp.15.016008
Si-Yang Jin 1 , Le-Ping Lin 2 , Xiao-Hui Chen 2 , Fan Liu 1 , Xiao-Bo Zhu 2 , Guo-Ping Yan 1
Affiliation  

In recent years, antibody-labeled fluorescent probes have attracted wide attention in fluorescence immunochromatography. Quantum dots (QDs) are excellent fluorescent materials with some unique optical properties, including strong stability against photobleaching, broad excitation spectra, high emission intensity, good fluorescence efficiency, and narrow excitonic emission. The CdSe/ZnS QDs microbeads were prepared by encapsulation in-situ of CdSe/ZnS QDs in polyisobornyl methacrylate (PIBOMA) herein and then functional QDs microbeads were produced by the binding of active carboxyl-modified QDs microbeads to the C-reactive protein (CRP) antibody. Their fluorescence properties were also investigated and functional QDs microbeads were further evaluated as fluorescent probes to detect different CRP concentrations using the fluorescence immunoassay test strips. The experiment data indicated that functional QDs microbeads possessed good stability, high fluorescence efficiencies, narrow fluorescence spectra, and good linear relationship of detection line signal versus quality inspection line signal (T1 / C) to the CRP concentration varying from 0.001 to 0.2 mg / l. Therefore, functional CdSe/ZnS QDs microbeads encapsulated in PIBOMA bioconjugated to CRP antibody can be used as the potential fluorescent probes to achieve quantitative detection in the immunochromatographic technology.

中文翻译:

免疫层析法原位包裹甲基丙烯酸冰片酯封装的荧光量子点微珠的制备及性能

近年来,抗体标记的荧光探针在荧光免疫色谱法中引起了广泛的关注。量子点(QD)是出色的荧光材料,具有一些独特的光学特性,包括对光漂白的强稳定性,宽激发光谱,高发射强度,良好的荧光效率和窄的激子发射。CdSe / ZnS QDs微珠是通过将CdSe / ZnS QDs原位封装在聚甲基丙烯酸异冰片酯(PIBOMA)中制备的,然后通过将活性羧基修饰的QDs微珠与C反应蛋白(CRP)结合来生产功能性QDs微珠。 )抗体。还研究了它们的荧光特性,并使用荧光免疫检测试纸进一步评估了功能性QDs微珠作为荧光探针,以检测不同的CRP浓度。实验数据表明功能性QDs微珠具有良好的稳定性,较高的荧光效率,较窄的荧光光谱,并且检测线信号与质量检测线信号(T1 / C)与CRP浓度在0.001至0.2 mg / l之间线性关系良好。 。因此,封装在与CRP抗体生物偶联的PIBOMA中的功能性CdSe / ZnS QDs微珠可以用作潜在的荧光探针,以实现免疫色谱技术中的定量检测。高荧光效率,窄荧光光谱以及检测线信号与质量检测线信号(T1 / C)与CRP浓度在0.001至0.2 mg / l之间的良好线性关系。因此,封装在与CRP抗体生物偶联的PIBOMA中的功能性CdSe / ZnS QDs微珠可以用作潜在的荧光探针,以实现免疫色谱技术中的定量检测。高荧光效率,窄荧光光谱以及检测线信号与质量检测线信号(T1 / C)与CRP浓度在0.001至0.2 mg / l之间的良好线性关系。因此,封装在与CRP抗体生物偶联的PIBOMA中的功能性CdSe / ZnS QDs微珠可以用作潜在的荧光探针,以实现免疫色谱技术中的定量检测。
更新日期:2021-02-25
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