The influence of different combinations of fresh fish and clam meat as feed on gonadal development and maturation in Amphiprion sebae was evaluated in a 60-day study. The study used four different combinations of diet viz. control diet (C: commercial feed with 50 % protein (C)), Treatment 1 (T1: fresh fish alone (F)), Treatment 2 (T2: fresh fish in combination with squid meat (FSQ)) and Treatment 3 (T3: fresh fish in combination with clam meat (FCM)). The experiment was conducted in triplicate, with Clown fish (mean weight: 15 g) stocked in tanks of 200 L capacity. The stocked fish were fed on alternate days with the respective assigned diets. On completion of the 60 days study period, maturation of the fish was assessed based on gonado-somatic index (GSI), fecundity and histology. The present study found highest GSI in fish fed with a diet of fresh fish and clam meat on alternate days (0.493 %), followed by fish fed with a diet of fresh fish and squid meat in alternate days (0.349 %) while the lowest GSI was observed in the group fed with the control diet (0.100 %). Average fecundity observed for A. sebae during the present study was 2204±137.4 eggs. Among the different diets, fish fed fresh fish and clam meat (T3) on alternate days had better gonadal development. On 60^th day, the ovary of T3 showed late vitellogenic oocytes with an appearance of yolk vesicles. Thus, it could be concluded that fresh fish in combination with clam meat can be used as maturation diet for marine ornamental fish A. sebae to attain early maturation in captivity.

中文翻译：

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鲜鱼和蛤肉作为成熟的饮食在Sebae小丑鱼，Amphiprion sebae（Bleeker 1853）中的应用

在一项为期60天的研究中，评估了新鲜鱼和蛤肉作为饲料的不同组合对两栖动物皮脂性腺发育和成熟的影响。该研究使用了饮食的四种不同组合。对照饮食（C：含50％蛋白质的商业饲料（C）），治疗1（T1：仅鲜鱼（F）），治疗2（T2：鲜鱼与鱿鱼肉组合（FSQ））和治疗3（T3） ：鲜鱼和蛤肉（FCM）组合。实验一式三份，将小丑鱼（平均重量：15 g）放到容量为200 L的水箱中。放养的鱼类每隔一天分别喂食指定的饲料。在为期60天的研究期结束后，根据性腺体细胞指数（GSI），繁殖力和组织学评估了鱼的成熟度。本研究发现，隔日喂新鲜鱼和蛤肉的鱼的GSI最高（0.493％），隔日喂新鲜鱼和鱿鱼肉的鱼的GSI最高（0.349％），而GSI最低的鱼对照饮食组中观察到（0。100％）。观察到的平均生殖力A. 鲷本研究期间为2204个±137.4鸡蛋。在不同的饮食中，隔天喂新鲜鱼和蛤肉（T3）的鱼的性腺发育更好。在^第60天，T3的卵巢显示出卵黄形成晚期卵母细胞，并出现卵黄囊泡。因此，可以得出结论，将新鲜的鱼和蛤肉一起用作海洋观赏鱼A. sebae的成熟饮食，以使其在圈养中早日成熟。