The plants belonging to the genus Embelia, a significant tropical genus with many biological activities, are benefiting because of their robust medicinal properties. Embelin is one of the principal bioactive molecules responsible for the medicinal properties of the genus Embelia. The quantification of the embelin compound among different species in this genus has not yet been investigated, so still uncertain which species and which part should be accepted. The present study was intended to establish a speedy and precise high-performance thin-layer chromatographic (HPTLC) method for quantitative study of embelin in various plant parts of Embelia ribes, Embelia tsjeriam-cottam, Embelia basaal, Embelia adnata, and Embelia gardneriana. This research confirmed the method as per the International Conference on Harmonization (ICH) guidelines. We achieved separation on silica gel 60 F254 HPTLC plates using propanol: butanol: ammonia (7:3:7 v/v/v) as a mobile phase. Densitometry scanning performed for detection and quantification at 254 nm and 366 nm. Among the species investigated, the highest amount of embelin was found in E. ribes fruits. Embelia ribes fruits are the best source of embelin. Embelin was first described in the endemic species, such as E. adnata and E. gardneriana. The method illustrated in this research may be applied for quantification of embelin and fingerprint analysis of other species within Embelia genus or described genera and chemo taxonomic studies of this genus.

中文翻译：

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Embelin：一种HPTLC方法，用于对5种Embelia Burm属进行定量估计。F。

属于Embelia属的植物，由于其强大的药用特性而受益匪浅，Embelia是具有许多生物活性的重要热带属。Embelin是负责Embelia属药用特性的主要生物活性分子之一。尚未研究该属中不同物种之间的栓塞化合物的定量，因此仍不确定应接受哪个物种和哪个部分。本研究旨在建立一种快速，准确的高性能薄层色谱（HPTLC）方法，用于定量研究Embelia肋骨，Esmilia tsjeriam-cottam，Emliaia basaal，Emliaia adnata和Embelia gardneriana的各个植物部位中的栓子。这项研究证实了根据国际协调会议（ICH）指南的方法。我们使用丙醇：丁醇：氨水（7：3：7 v / v / v）作为流动相在硅胶60 F254 HPTLC板上实现了分离。进行光密度测定法扫描以在254 nm和366 nm处进行检测和定量。在所调查的物种中，在肋骨大肠杆菌果实中发现了最高量的栓蛋白。el子果实是emb子的最佳来源。Embelin首先在特有物种，如E. adnata和E. gardneriana中得到描述。本研究中说明的方法可用于Embelia属中或该属的属和化学分类学研究中的Embelin定量和其他物种的指纹分析。在所调查的物种中，在肋骨大肠杆菌果实中发现了最高量的栓蛋白。el子果实是emb子的最佳来源。Embelin首先在特有物种，如E. adnata和E. gardneriana中得到描述。本研究中说明的方法可用于Embelia属中或该属的属和化学分类学研究中的Embelin定量和其他物种的指纹分析。在所调查的物种中，在肋骨大肠杆菌果实中发现了最高量的栓蛋白。el子果实是emb子的最佳来源。Embelin首先在特有物种，如E. adnata和E. gardneriana中得到描述。本研究中说明的方法可用于Embelia属中或该属的属和化学分类学研究中的Embelin定量和其他物种的指纹分析。