Lentil seeds have been packed in a dielectric barrier device and exposed for several minutes to a cold atmospheric plasma generated in helium with/without a reactive gas (nitrogen or oxygen). While no impact is evidenced on germination rates (caping nearly at 100% with/without plasma exposure), seeds’ vigor is clearly improved with a median germination time decreasing from 1850 min (31 h) to 1500 min (26 h), hence representing a time saving of at least 5 h. We show that the admixture of nitrogen to helium can further increase this time saving up to 8 h. Contrarily, we demonstrate that the addition of molecular oxygen to the helium discharge does not promote seeds’ vigor. Whatever the plasma chemistry utilized, these biological effects are accompanied with strong hydrophilization of the seed coating (with a decrease in contact angles from 118° to 25°) as well as increased water absorption (water uptakes measured 8 h after imbibition are close to 50% for plasma-treated seeds instead of 37% for seeds from the control group). A follow-up of the seeds over a 45-days aging period shows the sustainability of the plasma-triggered biological effects: whatever the plasma treatment, seeds’ vigor remains stable and much higher than for seeds unexposed to plasma. For these reasons, the seed-packed dielectric barrier device supplied with a He–N₂ gas mixture can be considered as a relevant dry atmospheric priming plasma in the same way as those used in routine by seed companies.

中文翻译：

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使用干燥的大气等离子底漆可持续改善种子活力：通过包衣的润湿性，吸水率和等离子反应化学的证据

小扁豆种子已包装在介电屏障设备中，并暴露于氦气中产生的冷大气等离子体中，该等离子体在有/没有反应气体（氮气或氧气）的情况下保持了几分钟。虽然没有证据表明对发芽率有影响（在有/无血浆暴露下，发芽率几乎达到100％），但种子的活力明显得到改善，发芽时间中位数从1850分钟（31小时）减少到1500分钟（26小时），因此代表至少节省5个小时的时间。我们表明，氮气与氦气的混合可以进一步增加这一时间，最多可节省8小时。相反，我们证明向氦气放电中添加分子氧不会促进种子的活力。不论等离子体化学如何利用，这些生物学效应伴随着种子包衣的高度亲水化（接触角从118°减小到25°）以及吸水率的增加（吸水后8小时测得的吸水率接近于等离子处理过的种子的50％）而不是对照组的种子的37％）。种子经过45天的老化期间的随访表明，血浆触发的生物效应具有可持续性：无论采用何种等离子体处理，种子的活力都保持稳定，并且比未暴露于血浆的种子要高得多。由于这些原因，装有He–N的种子填充介电阻挡层设备 种子经过45天的老化期间的随访表明，血浆触发的生物效应具有可持续性：无论采用何种等离子体处理，种子的活力都保持稳定，并且比未暴露于血浆的种子要高得多。由于这些原因，装有He–N的种子填充介电阻挡层设备 种子经过45天的老化期间的随访表明，血浆触发的生物效应具有可持续性：无论采用何种等离子体处理，种子的活力都保持稳定，并且比未暴露于血浆的种子要高得多。由于这些原因，装有He–N的种子填充介电阻挡层设备_2种气体混合物可以被视为相关的干燥大气引发等离子体，与种子公司常规使用的方法相同。