Triclosan is a chlorinated phenolic, used in many personal and home care products for its powerful antimicrobial effect. Several studies have shown triclosan toxicity and the American Food and Drug Administration (FDA) in 2016 has limited its use. It has been recently included in endocrine-disrupting chemicals (EDCs), a list of chemicals known for their ability to interfere with hormonal signaling with particular critical effects on reproduction both in animals and humans. In order to deepen the knowledge in this specific field, the present study was undertaken to explore the effect of different concentrations of triclosan (1, 10, and 50 µM) on cultured luteal cells, isolated from swine ovaries, evaluating effects on growth Bromodeoxyuridine (BrDU) incorporation and Adenosine TriPhosphate (ATP) production, steroidogenesis (progesterone secretion) and redox status (superoxide and nitric oxide production, enzymatic and non-enzymatic scavenging activity). A biphasic effect was exerted by triclosan on P4 production. In fact, the highest concentration inhibited, while the others stimulated P4 production (p < 0.05). Triclosan significantly inhibited cell proliferation, metabolic activity, and enzymatic scavenger activity (p < 0.05). On the contrary, nitric oxide production was significantly increased by triclosan (p < 0.01), while superoxide anion generation and non-enzymatic scavenging activity were unaffected.

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三氯生对培养的猪黄体细胞的作用评估

三氯生是一种氯化酚，由于其强大的抗菌作用而用于许多个人和家庭护理产品中。多项研究表明三氯生具有毒性，美国食品药品监督管理局（FDA）在2016年限制了其使用。最近，它已被包括在内分泌干扰化学物质（EDC）中，该物质以其干扰激素信号传递的能力而闻名，对动物和人类的生殖具有特别重要的影响。为了加深该领域的知识，本研究旨在探索不同浓度的三氯生（1、10和50 µM）对从猪卵巢分离的黄体细胞的培养作用，评估其对溴脱氧尿苷（ BrDU）的掺入和三磷酸腺苷（ATP）的生产，类固醇生成（孕酮分泌）和氧化还原状态（超氧化物和一氧化氮的产生，酶促和非酶促清除活性）。三氯生对P4产生双相作用。实际上，最高浓度会抑制，而其他浓度会刺激P4的产生（p＜0.05）。三氯生显着抑制细胞增殖，代谢活性和酶清除剂活性（p <0.05）。相反，三氯生可显着增加一氧化氮的产生（p <0.01），而超氧阴离子的产生和非酶清除活性均不受影响。