The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

中文翻译：

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双色高粱发芽过程中的HPLC-DAD多酚分析及抗氧化活性

这项研究的目的是评估罗马尼亚高粱双色Albanus杂交种的适宜性。作为潜在的功能性食品成分。在不同的发芽期（24、36和48小时）之前和期间，使用不同溶剂的超声辅助萃取，分光光度法和色谱法来监测总酚和类黄酮含量的变化以及粗高粱籽粒的抗氧化活性。提取物的高效液相色谱-二极管阵列检测（HPLC-DAD）研究表明，甲醇提取的未发芽高粱籽粒中酚类化合物的多样性最高，而总酚含量（TPC）在36之后最高。在两种提取物中的h发芽时间：甲醇提取物为1853 mg GAE / 100 g，乙醇提取物为1726 mg GAE / 100 g。高粱双色谷物。