Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.

中文翻译：

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有机染料的光致蓝化可能会导致超分辨率显微镜中的伪影

荧光团的照射会导致荧光能力丧失，称为光漂白。有趣的是，一些荧光团光转换为蓝移荧光分子作为光漂白途径的中间体，这会使多色荧光成像复杂化，特别是在超分辨率荧光成像中使用的强激光照射下。在这里，我们讨论荧光团光蓝化的机制及其对荧光成像的影响，并展示如何预防它。