Serotonin (5-HT) is an important signaling monoamine and neurotransmitter. We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick Argas monolakensis. G-GESS shows fast response kinetics and high affinity, specificity, brightness and photostability. We used G-GESS to image 5-HT dynamics in cultured cells, brain slices and behaving mice.

中文翻译：

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撤回文章：一种快速、高亲和力的荧光血清素生物传感器，由蜱脂质运载蛋白设计而成

血清素 (5-HT) 是一种重要的信号单胺和神经递质。我们报告了一种绿色荧光、基因编码的血清素传感器 (G-GESS) 的结构引导工程，该传感器来自软蜱Argas monolakensis中的 5-HT 结合脂质运载蛋白。G-GESS 显示出快速响应动力学和高亲和力、特异性、亮度和光稳定性。我们使用 G-GESS 对培养细胞、脑切片和行为小鼠中的 5-HT 动力学进行成像。