Atmospheric Hg is a highly toxic heavy metal with bioaccumulative properties. However, relatively few studies have focused on the distribution of Hg in cellular and subcellular structures of plants and factors influencing its accumulation. In this study, we selected Tillandsia usneoides, which is a widely used bioindicator for Hg, to analyze the concentration of Hg in different cells (foliar trichomes, epidermal cells, mesophyll cells, and vascular bundle cells), different subcellular structures (cell wall, cell membrane, vacuoles, and organelles) and different cell wall components (pectin, hemicellulose 1, and hemicellulose 2). It was determined that Hg was present in different types of cells, but there was no significant difference, suggesting that atmospheric Hg circulates dynamically in the surface and internal structural cells of T. usneoides leaves. Subcellular analysis showed that as Hg concentration increased, more Hg accumulated in the vacuoles and cell wall through the compartmentalization mechanism. Hemicellulose had the highest content of Hg, indicating that it is the primary Hg-binding component of the cell wall. The FTIR analysis results showed that after the Hg treatment, the cell wall -OH and COO- absorption peaks changed most significantly, indicating that these functional groups play a vital role in the Hg accumulation process.

大气汞是具有生物蓄积特性的剧毒重金属。然而，相对较少的研究集中在汞在植物细胞和亚细胞结构中的分布以及影响其积累的因素。在这项研究中，我们选择了铁兰（Tillandsia usneoides），它是汞的一种广泛使用的生物指示剂，可分析不同细胞（叶毛，表皮细胞，叶肉细胞和血管束细胞），不同亚细胞结构（细胞壁，细胞膜，液泡和细胞器）中的汞浓度，并不同的细胞壁成分（果胶，半纤维素1和半纤维素2）。可以确定，汞存在于不同类型的细胞中，但没有显着差异，表明大气中的汞在T. usneoides的表面和内部结构细胞中动态循环。树叶。亚细胞分析显示，随着Hg浓度的增加，更多的Hg通过隔室机制在液泡和细胞壁中积累。半纤维素具有最高的Hg含量，表明它是细胞壁的主要Hg结合成分。FTIR分析结果表明，汞处理后，细胞壁-OH和COO-吸收峰变化最大，表明这些官能团在汞积累过程中起着至关重要的作用。